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Currently, the diagnosis of SARS-CoV-2 infection includes detection of key viral genes RT-PCR. In addition, in situ detection of key virus-host interaction genes and analysing immune response from the host are key to better understand and improve existing diagnostic and treatment regime for SARS-COV-2.

Quantitative synthetic molecular standards (BSL1) to detect and quantify SARS-CoV-2

When developing and using a novel detection assay, researchers need access to clinically relevant positive controls to ensure the reliability and accuracy of their results. ATCC has developed three quantitative synthetic molecular standards (BSL1) to detect and quantify SARS-CoV-2, as well as Coronavirus strains and nucleic acids from these strains. It includes SARS-CoV-2 construct compatible with PCR primer and probe sets developed by the CDC (Cat No VR-3276SD), and heat-inactivated SARS-CoV-2.

ATCC Synthetic Molecular Standards for SARS CoV 2


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SARS-CoV-2 Molecular Diagnostics Development

Reliable Diagnostic Tools Depend on Credible Reference Materials. For commercial manufacturers developing SARS-CoV-2 molecular diagnostic tests for emergency use, the FDA has provided recommendations for validation studies to be conducted. To support this critical research, ATCC provides a variety of authenticated and clinically relevant tools.

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Direct visualization of the virus and estimate the viral load in cells

RNAscope technology allows for direct visualization of the virus and estimate the viral load in cells (Any Gene, Any Species, Any Tissue) with morphological context. Whether it is detecting the SARS-CoV-2 spike protein (Cat No 848561 V-nCoV2019 –S), and combine it with probes for the cellular receptor (ACE2) and proteases (TMPRSS2, Cathepsin B and Cathepsin L) or monitoring inflammatory response in cells (IL-6, IL-1β, IL-10 and TNFα), RNAscope technology allows spatial resolution of biomarkers in a quantitative manner. 

Detection of positive single-stranded RNA virus

ACD Bio Detection of positive single stranded RNA virus B

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Custom Services

Molecule animation SMWe understand everyone’s research is unique. If you cannot find the molecular construct or nucleic acids required for your research, or you need a customised RNAscope probes or you want to use an existing antibody for an untested application, please contact our team at


Novus Biologicals and R&D Systems provide a broad range of antibodies compatible with IHC, ICC and IF. These can be combined with RNAscope porbes to perform Dual ISH-IHC. Thus, the application is extended to include not only viral targets and host immune response targets, but also detect key biomarkers specific to host cells. 

ACE2 detection BiotechneACE‑2 detection in Human Kidney using a Goat Anti-Human ACE‑2Antigen Affinity-Purified Polyclonal Antibody (R&D Systems, Catalog # AF933) and Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R&D Systems, Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

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New Publication alert

This publication cites R&D Systems Ace2 polyclonal antibody as showing the capacity to block Novel Coronavirus virus entry.  
> Read the publication now
> Find out more about the Ace2 polyclonal antibody

Coronavirus Research Resources

Immunology Serology NPTherapeutic Development NPDrug repurposing NP

Vaccine Development NP

Together, we can discover the tools needed to contain the spread of this pathogen.

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