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Upcoming Webinars/Events

single cell

Optimizing Single-cell Dispensing for Microbial Research

Date: June 2, 2021

Time: 4:30pm AEST
 
Description: A fully anaerobic cultivation workflow for high-throughput isolation of gut bacteria
Automated and label-free cloning of coli and yeast. Genome sequencing of uncultivated bacteria in the human oral microbiome. Using the B.SIGHT™ single-cell dispenser for automated and label-free isolation of bacteria, archaea and yeast

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Previous Webinars/Events

Luminex Webinar May 2021 LinkedinDeep Dive: Luminex Multiplex Assays

Date: Wednesday 19th May.

Time: 2.00pm AEST & 12.00pm NZ.

Description: Multiplexing is key for screening various biomarkers for the identification of diseases. During this webinar Senior Bio-Techne Scientist, Richard Fuerstenburg, will walk you through the validation needed for Luminex assays. You’ll also learn about the factors that provide specificity and sensitivity to our Bio-Techne assays.

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Radleys IVT Webinar Linkedin

"Why you might be doing your small-scale process development wrong"

Join us for the latest Webinar 

Date: Wednesday 12th and Thursday 13th May.

This 15 minute webinar is offered at 5 different times to suit all time zones.

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Day of Immunology Webinar Page PanelWe are proud to be an ongoing supporter of the Australia and New Zealand Society for Immunology (ASI). 

Join us virtually to celebrate International Day of Immunology with an expert panel discussion on COVID-19 vaccines

When:
Fri, 30 April 2021

Time:
5:30 PM – 7:30 PM AEST

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2550 Celvivo Linkedin Webinar 2












CelVivo Webinar

"Why active diffusion and low shear forces makes a difference when cultivating cells in 3D”

Join us for the first webinar from CelVivo, titled, “Why active diffusion and low shear forces makes a difference when cultivating cells in 3D”

Date: Tuesday 22nd of April 4PM AEST.

Please Register in advance for this webinar

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Please send your Questions in advance to jsv@celvivo.com and they will be answered during the session.


2562 BUCHI Science Week Linkedin

 

 

 

 

 

 

 

BUCHI Science Week

BUCHI Science Week 20/4/2021 - 22/4/2021 Don't Miss Out!
Sweeten up your day and get rewarded with vouchers and a mystery gift with full attendance of at least 1 session!


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CGT webinar DW Social APAC Webinar 550x272 8APR2021

Tools for Cell Based Therapies

When: 8th April 2021

Time:
2pm AEST Australia 
4pm New Zealand

Speakers:

• Nithya Jesuraj, Ph.D. (Product Manager, Genome Engineering, Bio-Techne)
• Kevin Flynn Ph.D. (R&D Manager, Stem Cells, Bio-Techne)
Description:
IN VITRO TECHNOLOGIES and BIO-TECHNE IS DELIGHTED TO INVITE YOU TO ATTEND OUR LIVE WEBINAR.
We are bringing together Bio-Techne experts across disciplines within Cell and Gene Therapy to present and explore:

  • Optimizing ex vivo Stem Cell models for studying development, disease and drug discovery
  • Cell and gene therapy workflow solutions for Immunotherapies

We are excited to provide this forum to discuss these key topics with 2 key speakers and a Q&A/Panel session.

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ATCC EDM Panel

ATCC logo website

Expand Your Cell-based Assays with an Unlimited, Biologically Relevant Resource

When:
Friday 26 March , 2021

Time:
12:00 noon - 1 PM AEST
10:00-11:00 AM New Zealand

Discover ATCC’s range of differentiated iPSCs cells for toxicology & cancer research.

Toxicology and cancer researchers alike are challenged by the lack of a consistent source of cells with high physiological relevance for their cell-based assays. The source tissue for primary cells comes from a variety of donors, which can introduce interexperimental variability and confound experimental results. Differentiated induced pluripotent stem cell (iPSC)-derived cells fulfill the need for a consistent source of cells and physiological relevance. iPSCs are able to be generated in large cell numbers from a single clone, providing the needed consistency. Further, iPSCs can be differentiated into a variety of desired, functional cell types, affording the necessary biological relevance. Here we provide data demonstrating how differentiated iPSC-derived cells can be incorporated into immunoassays or further differentiated into cell types such as osteocytes, chondrocytes, and adipocytes.

Presented by:

Brian Shapiro, PhD, Scientific Content Specialist, ATCC

Brian A Shapiro, Ph.D., works to communicate the scientific breakthroughs of ATCC’s product development laboratories to the biomedical research community. Previously, he worked at Virginia Commonwealth University, where he investigated the role of pre-mRNA splicing in the multi-drug resistance of lung cancer. Dr. Shapiro attended the Medical College of Georgia, where his research focused on adrenal physiology as well as diseases of the epidermis.

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ACD Bio APAC Webinar Panel 002

Oncofetal Ecosystem: Space, Time and Memory

Join Advanced Cell Diagnostics & In Vitro Technology for this exciting Nature Research (Publishing) webinar on

When: Wed March 17, 2021

Time: 12PM AEST

Speaker: Ankur Sharma, Ph.D., Harry Perkins Institute of Medical Research and Curtin University, Perth, Australia

Description: 

This webcast will describe employing single cell genomics and spatial transcriptomics to discover a previously unexplored oncofetal reprogramming of the tumor ecosystem.

Analysis of ~212,000 cells representing human fetal, hepatocellular carcinoma (HCC), and mouse liver revealed remarkable fetal-like reprogramming of the tumor microenvironment. Specifically, the HCC ecosystem displayed features reminiscent of fetal development, including re-emergence of fetal-associated endothelial cells and fetal-like tumor-associated macrophages.

The speaker will describe a shared immunosuppressive oncofetal ecosystem in fetal liver and HCC and its potential implications for targeting therapeutic interventions in HCC and identifying similar paradigms in other cancers and diseases.

You will learn:

•            Identification of fetal-associated endothelial cells and macrophages in HCC

•            Shared oncofetal ecosystem between human fetal liver and HCC

•            The phenomenon of oncofetal reprogramming in the tumor ecosystem

 

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CGTEvent








ISCT ANZ Regional Virtual Meeting

Virtual Conference:

Friday, February 26, 2021

ISCT is a global society of clinicians, regulators, technologists, and industry partners with a shared vision to translate cellular therapy into safe and effective therapies to improve patients' lives. The ISCT ANZ 2021 Virtual Regional meeting is scheduled for 26th of February 2021. In Vitro Technologies are proud to support this event as a sponsor and will have a virtual booth to exhibit our Cell and Gene Therapy (CGT) portfolio. The team at In Vitro Technologies can help you at all stages of CGT Research: from discovery to the clinic. Our high quality reagents and consumables will help ensure confidence in your data, while our closed-automation equipment will help achieve critical challenges: scalability and manufacturability. Register Now! . 

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Eradicate Cancer Virtual EDM









Eradicate Cancer: Next Frontiers in Immunotherapy

Virtual Conference:

December 15-16th, 2020

This conference brings together the necessary ingredients to create a meaningful immunotherapeutic platform to attack cancer and step down the path to potentially cure it. The 2020 Eradicate Cancer will be a virtual conference (15-16th  December 2020)  There will be an outstanding faculty of speakers at Eradicate Cancer 2020. Register Now! . 

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Bioprocess: Why standardized cell counting is critical to assess viability and concentration

Webinar:

Wednesday, October 21st, 2020  11.00AM In Paris. 

Among challenges in bioprocessing, cell counting is one of the most critical. Standardization of the cell counting method to assess cell concentration and viability requires careful selection. Choice of the method, accuracy and precision of the devices, throughput and compliance are some of the factors which can impact the success of cell production. 

Join us to learn about our most powerful cell counter, the LUNA-FX7™

Presented by:

Karine Labour
 

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Milk the most out from Dairy analysis with NIR technology

Webinar:

Wednesday, October 21st, 2020 from 14.00 - 14.30 AEDT.

High quality milk products can only be produced from high quality raw milk, so a close quality control along the milk processing chain is vital - from the intake at the dairy plant, to the various processing steps, and the end products. This live webinar allows you to learn about the latest near infrared spectroscopy (NIR) technology, how to make use of it to improve food quality, safety and process control monitoring.

Presented by:

Ms. Nattakarn Navasearttavisootr

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Bio-Techne ANZ miRNAscope Webinar

Visualize ASO, miRNA and siRNA Detections with Spatial Resolution

When:

Tuesday, September 22nd 2020
11:00 A.M to 12,30PM. Australian Eastern Standard Time

Outline:

Get a deeper understanding of gene expression patterns by using assays that retain spatial organization at single cell resolution! Come learn about the new robust RNA in situ hybridization assay that enables the detection of ASOs, miRNAs, siRNAs, and other smaller RNAs (16-50nt) with spatial and morphological context at single cell resolution.
  1. Introduction to the miRNAscope technology and workflow
  2. Key applications that necessitate use of the miRNAscope Assay
  3. Key benefits of the miRNAscope Assay for your research
 

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2385 LS Biotechne Webinar Image V1






COVID-19 Symposium: Research Tools From Bio-Techne 

When:
Monday, August 26th, 2020
1:00 P.M. Australian Eastern Standard Time
                                  

Coronavirus research is picking up pace across the world but due to social distancing measures tools that provide accurate results in minimum hand on time are the need of the hour. Bio-Techne has developed many tools to help researchers study and fight the virus that will be coverd today. This symposium is divided into four talks, beginning with a discussion on COVID-19 related proteins in the first session followed by antibodies, analytical tools for serology and small molecules and ISH assay.                     

Presented By:

Anthony Person, Senior Director, Proteins at Bio-Techne

Steve Orstad, Antibodies at Bio-Techne

Annegret Boge Product Manager Bio-Techne

Suruchi Arora Product Manager Bio-Techne

 

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terumo webinar






COVID-19 experts discuss exosomes and mesenchymal stem cells as experimental treatment for ARDs in COVID-19

terumo logo

 

When:
Friday, June 5, 2020
1:00 A.M. Australian Eastern Standard Time
3:00 A.M. New Zealand   

Also available on Demand                                     

Join us for this timely panel discussion that will cover ongoing research and clinical trials to manage acute respiratory distress syndrome (ARDS) in COVID-19 patients. Two experts will share their extensive experience with therapies using mesenchymal stem cells (MSCs) and the small extracellular vesicles (sEVs) that MSCs produce as potential treatments for COVID-19 patients with ARDS. Jim Beltzer, PhD, from Terumo BCT will facilitate the discussion.11:00 a.m. Eastern Time, USA                                   

In this webinar, we will:

Explore how small extracellular vesicles can recapitulate the therapeutic effect of MSCs
Discuss clinical trial design with critically ill patients (such as those with COVID-19), including strategies for optimizing sample size, managing patient and disease heterogeneity, and maximizing the use of trial data
Learn how the lung inflammatory environment may alter mesenchymal stem cell behaviour

Presented By:

Ben Buller, PhD, Associate Scientist, Henry Ford Health System

Michael Matthay, MD, Professor of Medicine, University of California San Francisco

Jim Beltzer, PhD, Senior Manager, Terumo BCT

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ACD Biotechne CMYK

RNAscope™ Technology Introduction: How to get started as a new user

When:
Thursday, April 30, 2020
11:00 A.M. Australian Eastern Standard Time
1:00 P.M. New Zealand

Outline:
The RNAscope® Technology is a novel in situ hybridization (ISH) assay for detection of target RNA within intact cells. The assay represents a major advance in RNA ISH approaches with its proprietary probe design to amplify target-specific signals but not background noise from non-specific hybridization. The RNAscope® Assay is an ideal tool to complement technologies like IHC, qPCR or NGS. In this webinar we will discuss the benefits of RNAscope® assay and share tips and tricks on how to perform these assays successfully to obtain publication-quality RNA in situ results. Please bring your RNAscope® technology questions to the webinar and our team will answer them live during the webinar.
 
Who should attend:

This webinar will benefit both new and experienced RNAscope® technology users.

Learning Objectives:
  • How RNAscope® assays work
  • What you need to get started
  • Tips and tricks for successful sample preparation
  • Examples of staining results & research application specific data
  • FAQs & Troubleshooting tips

Presented By:
Amrita Roy, PhD
Field Application Scientist
Advanced Cell Diagnostics, Bio-Techne

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ATCC EDM Panel

ATCC logo website

Amplify Your Viral Vaccine Production with CRISPR/Cas9-Engineered Host Cells

When:
Thursday, April 23, 2020
11:00 A.M. Australian Eastern Standard Time
1:00 P.M. New Zealand

Vaccines against viral infections like polio, chickenpox, measles, and viral hepatitis are manufactured by producing large quantities of viral particles in a scaled-up tissue culture system. A handful of historical cell lines like Vero (green monkey kidney) and MDCK (canine kidney) are approved by the FDA for viral vaccine production. However, other than clone selection and substrate adaption, relatively little has been done to improve the inherent viral production capacity of the cell lines. ATCC used CRISPR/Cas9 gene editing to create improved versions of cell lines commonly used for viral vaccine manufacturing. These cells produce model
clinical viruses at titers 10-fold higher than the corresponding parental cell line, which has the potential to significantly reduce costs associated with virus and viral vaccine production.

In this webinar, ATCC Scientist Dr. Liz Gillies will cover these key takeaways:

  • Most viral vaccines are manufactured in large-scale tissue culture systems using historical cell lines that are approved for vaccine manufacturing.
  • ATCC used CRISPR/Cas9 gene-editing techniques to increase the viral production efficiency of some of these historical cell lines.
  • These enhanced virus-producing cell lines have the potential to significantly reduce the costs associated with generating viral vaccines and high-titer viral stocks.

Presented by:

Liz Gillies, Ph.D., Scientist, ATCC

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/brands/agilent-technologies-seahorse-bioscience/

Combining Seahorse XF Analysis with stable isotope tracing to reveal novel metabolic drug targets

In this webinar, Dr. David Ferrick discusses how advances in our understanding of T cell co-receptors have led to new strategies for keeping immune cells turned on in the suppressive, hostile tumor microenvironment. Much has been learned in the last decade about metabolic reprogramming in cancer, focused initially on tumor metabolism. However, by applying this knowledge to infiltrating immune cells we are now beginning to appreciate the metabolic drivers of immune cell fitness, specifically stable mitochondrial function that can perhaps reverse exhaustion phenotypes by enhancing survival and function in hypoxic and nutritionally depleted tumor microenvironments.

Presented by:

David Ferrick, Ph.D, 

Senior Strategy Director 

Cell Analysis, Agilent

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/brands/agilent-technologies-seahorse-bioscience/

Join us for out eSeminar presented by Dr. Ping-Chih Ho to learn about Agilent Cell Analysis applications in cancer immunotherapy research

The aberrant accumulation of regulatory T cells (Tregs) in tumors promotes immunosuppressive features of the tumor microenvironment (TME). Although depleting Tregs unleashes antitumor immunity and reprograms the immune status of the TME, the therapeutic potential of Treg-targeting is largely compromised due to autoimmunity induced by systemic loss of Tregs. Given that Tregs reside in different locations where they contain distinct nutrient composition, metabolic adaptation should be engaged by Tregs to support their tissue retention and tissue-dependent behavior. This talk discusses how we can selectively demolish intramural Tregs by targeting unique metabolic preference of intratumoral Tregs and how this approach can be utilized for cancer research.

Presented by:

Ping-Chih Ho, Ph.D,

Assistant Professor,

Department of Fundamental Oncology,

University of Lausanne Ludwig Center for Cancer Research.

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cell analysis seahorse webinar

 

Rapid adoption of live cell kinetics and real time biology in the design and validation of adoptive T cell strategies



Adoptive cell therapies have come of age, establishing living cells as a powerful therapeutic modality in terms of specificity, durability and adaptability. Agilent Seahorse technology is enabling the shift to real-time quantification of immune cell function, opening a new window on controlling immune cell fate and outcomes. Several translational research studies have demonstrated that altering metabolic behavior can direct immune responses and outcomes. Move beyond analyzing what your cells are, and reveal a clearer picture of what they do in order to harness their therapeutic potential.

David Ferrick, Ph.D

Senior Director

Cell Analysis Division - Agilent Technologies

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ATCC logo website

STR Profiling for Human Cell Line Authentication

Over the past few decades, much has been written about misidentification of cell lines, primarily human cell lines. It is not unusual that studies of the ‘same’ cell lines performed by different laboratories often show different results using the same methodology. Hear Dr. Shapiro discuss the implementation of best tissue culture practices to include routine authentication of human cell lines by short tandem repeat (STR) profiling.

Presented by:

Brian Shapiro, Ph.D. Technical Writer, ATCC

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ATCC logo website

The Art and Science of Growing Animal Viruses in the Laboratory: Best Practices in Virus Culture

Employing good aseptic technique and understanding the specific culture requirements of a strain are essential for optimal viral growth. In this webinar, we will discuss the basic principles for propagating animal viruses in the laboratory. Here, we will highlight best practices for making good virus stocks with an emphasis on the art and science of virus culture that should be followed to help avoid contamination and cross-contamination. Further, we will provide an in-depth look at viral culturing practices in both cell culture and embryonated chicken eggs using Influenza virus as an example.

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Agilent

Quantifying macrophage activation using Agilent Seahorse XF technology

Metabolism is critical for normal macrophage function - upon activation there is a dramatic metabolic switch towards glycolysis.  In this webinar we will discuss new approaches for quantifying macrophage activation in real time using an Agilent XF analyzer. 

Presented by:

Brian Dranka, Ph.D.

Biology Manager, Seahorse XF Products, Agilent Technologies, Inc.

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ATCC logo website

New isogenic cell models created by CRISPR genome editing for drug discovery

The recent development of the CRISPR/Cas9 system provides a revolutionary gene-editing technology. It is not only an advanced tool for basic research in biology but also a breakthrough technique for the development of targeted cancer therapies.

Presented by:

Fang Tian, Ph.D., Lead Scientist, ATCC Cell Systems

Lysa-Anne Volpe, M.S., Senior Biologist, ATCC Cell Systems

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Agilent

Metabolic reprogramming in macrophages during inflammation

Macrophages undergo profound metabolic changes when they are being activated. A major event is a switch to glycolysis which occurs in response to the bacterial product LPS. 

Presented by:

Luke O’Neill PhD FRS

Professor of Biochemistry, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin

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ATCC logo website

Cell Health and Viability

Assessing cell viability and cell health is crucial to all aspects of cell culture. In addition to the proper maintenance of cell stocks, cell health practices increase the level of confidence in experimental results.

Presented by:

Steven Budd, M.S., MBA Product Line Business Specialist, ATCC Cell Systems

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ACD Biotechne CMYK

Optimize Your RNAscope® Assay Data

In this webinar we present best methods to analyze and present RNAscope® assay data.

Presented by:

Annelies Laeremans - Ph.D. Scientist, Advanced Cell Diagnostics, Inc.

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Agilent

Type 1 Interferons Induce Change in Core Metabolism that are Critical for Immune Function

Presented by:

Duojiao Wu, M.D., Ph.D. - Associate Professor in Zhongshan Hospital, Fudan University, Principle Investigator and Director of Systems Immunology. Group/Platform in Shanghai Institute of Clinical Bioinformatics

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Agilent

Systems-based approach to characterising human pluripotent stem cell metabolism

Presented by:

Hui (Sunny) Zhang - Research Professor. Zhongshan Medical School, Sun Yat-sen University in Guangzhou, China

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Agilent

Immunometabolism

This webinar will explore the intricate links between immunology and metabolism.

Presented by:

Lydia Lynch - Associate Professor. Trinity College Dublin Instructor, Harvard Medical School

Christoph Hess - Professor of Medicine. University Hospital Basel

Nicholas Restifo - Senior Investigator. National Cancer Institute

Moderator: Anne Granger - Scientific Editor, Cell Metabolism

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Agilent

Working Towards Defining a Metabolic Roadmap for Stem Cell Lineage Progression

Learn why metabolism is important – think beyond cellular energetics and how metabolism changes in different stem cell populations (eSCs, iPSCs and adult SCs).

Presented by:

Dr. James Ryall

Department of Physiology

The University of Melbourne

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ACD Biotechne CMYK

The Next Generation RNA In Situ Hybridization Technologies

In Vitro Technologies are proud to invite Dr Yuling Luo, President of Advanced Cell Diagnostics to present a seminar series on Next Generation RNA In Situ Hybridization Technologies For Research and Diagnostics.

Presented By:

Dr Yuling Luo

President of Advanced Cell Diagnostics

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ATCC logo website

ATCC Microbiology Webinar - Best Practices for Stock Maintenance

Join us on Thursday, May 18 to hear Ms. Carol Horton discuss the best practices for stock maintenance with regard to passage, storage, recovery, and microbial authentication, and how ATCC manages these through the seed stock concept, adhering to the specific needs of each culture, and polyphasic strain authentication.

Presented by:

Carol Horton, M.S.

Marketing Manager, ATCC

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ACD Biotechne

RNAscope® Assay Data Analysis

Getting good conclusive results from your experiments begins with accurate analysis of your data. In this webinar, we will focus on the RNAscope® Assay and the following topics:

  • Review RNA ISH images with poor, medium and high quality targets
  • RNA ISH scoring tips RNA ISH data in comparison with IHC
  • Discuss a specific data set for HPV detection in FFPE tissue samples

Presented by:

Dr. Nancy Ciau

Board Certified Pathologist,

Advanced Cell Diagnostics, Inc.

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rnd logo

Next Generation Targets for Immuno-Oncology

Please join us for our webinar on Next Generation Targets for Immuno-Oncology. This webinar will discuss novel immunoregulatory pathways that we have uncovered as potential targets for oncology research and cancer immunotherapy.

Speaker:

Anthony Person, PhD

Manager in Bioassay Development

Bio-Techne

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atcc webinar

Cell Line Contamination – Mycoplasma Prevention and Detection

Mycoplasma infection can deprive host cells of nutrients and induce global changes in host gene expression, which in turn can affect data reproducibility and lead to the misinterpretation of experimental results. Best practices in cell culture coupled with routine testing is critical for the prevention and early detection of mycoplasma contamination.

Key points:

  • Mycoplasma contamination significantly affects cell line physiology, resulting in erroneous data and misinterpretation of results.
  • Laboratory best practices and routine testing are essential for minimizing mycoplasma contamination.
  • ATCC provides all three of the common methods of mycoplasma detection, including direct culture, Hoechst DNA staining, and PCR-based testing.

When:

Thursday, September 20, 2018
2:00 am Tuesday, in Sydney NSW


Presented by:

Reed Shabman, Ph.D.,
Lead Scientist, ATCC Standards Resources Center

Balsam Shawky, M.S.,
Senior Biologist, ATCC Standards Resource Center

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/brands/agilent-technologies-seahorse-bioscience/

Agilent Seahorse Webinar - Cell Analysis
Exploring the metabolism of CAR-T cells

In this webinar we will discuss:
- How CAR-T cells containing 4-1BB signaling domains have enforced in vitro persistence
- 4-1BB-enforced metabolic reprogramming enhances oxidative metabolism
- Mitochondrial biogenesis is selectively induced in 4-1BB CAR-T cells.

Presented by:

Roddy O'Connor, PhD

University of Pennsylvania

Center for Cellular Immunotherapies

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/brands/agilent-technologies-seahorse-bioscience/

Combining Seahorse XF Analysis with stable isotope tracing to reveal novel metabolic drug targets

This webinar will discuss how merging quantitative, rate-based measurements of oxygen consumption with detailed pathway analysis using mass spectrometry have revealed the mitochondrial pyruvate carrier (MPC) as a central regulator of metabolic flexibility.

Presented by:

Ajit Divakaruni - Assistant Professor, Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA)

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ACD Biotechne

Get the most out of your RNAscope® Multiplex Fluorescent assays

Get the most out of your RNAscope® Multiplex Fluorescent assays

RNAscope® Multiplex Fluorescent assays are ideal for single-molecule detection of multiple RNA targets simultaneously within the same tissue. They are very powerful in identifying co-expression or co-localization of RNA within specific sub-populations of cells.

Presented by:

Ming Xiao He, Ph.D, Scientist, Marie Lauigan Technical Support Scientist.

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AgilentiPSC-derived neural cells for drug discovery of mitochondrial disorders

Mutations in mitochondrial DNA (mtDNA) cause diseases typically affecting the nervous system and for which no effective treatment exists.

Presented by:

Alessandro Prigione, M.D, Ph.D.

Independent Team Leader

Max Delbrueck Center for Molecular Medicine (MDC) in Berlin

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ATCC logo website

Human Renal Proximal Tubule Epithelial Cells (RPTEC/TERT1) Modified to Express Drug Transporters - a New Model for Toxicity Studies

In vivo studies have shown that kidney membrane transporters play a key part in drug disposition and renal clearance. Primary renal proximal tubule epithelial cells (RPTEC) are the most physiologically relevant cell models, but lose OAT1 and OCT2 transporter expression in culture.

Presented by:

Chaozhong Zou, Ph.D.

Senior Scientist,

ATCC Cell Systems

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ACD Biotechne

Tips & Tricks to Get Publication Quality RNA ISH Data for Neuroscience Applications

RNAscope® Technology is a novel in situ hybridization (ISH) assay for detection of target RNA within intact cells. It represents a major advancement in RNA ISH approaches. 
In this webinar we will discuss the benefits of both RNAscope® and BaseScope™ assays and share tips and tricks on how to perform these assays successfully to obtain publication-quality RNA in situ results.

This webinar will benefit both new and experienced RNAscope® technology users.

Presented by:

Nidhi Vashistha, Ph.D

Technical Support Scientist

Advanced Cell Diagnostics, Inc

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ATCC logo

Advanced Models of Parkinson's Disease

Optimized cell lines are essential for modelling neurodegenerative diseases, screening novel therapeutics, and testing the neurotoxicity of environmental compounds.  Neural progenitor cells (NPCs) derived from induced pluripotent stem cells are excellent in vitro models as they can be induced to differentiate down all three neural lineages.

Speaker: 

Brian Shapiro, Ph.D. 

Technical Writer, ATCC

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/brands/agilent-technologies-seahorse-bioscience/

Metabolic switches that drive stem cell specification and differentiation to a mature neuron

As pluripotent stem cells become specified towards a neural stem/progenitor cell and then further differentiate into a mature neural cell type, they go through a number of molecular and metabolic changes that shift the balance between glycolysis and oxidative phosphorylation. Through the differentiation process stem cells turn off their dependence on glycolysis and move towards oxidative phosphorylation. However, whether the lineage specific gene expression drives the metabolic switch or vice versa remains unknown and is an aspect the we will further explore in this webinar.

Presented by:

Eva Szabo MSc, PhD

Canada Research Chair in Metabolism in Human Stem Cells and Cancer Development

Principal Investigator - Stem Cell and Cancer Research Institute (SCC-RI)

Assistant Professor - Biochemistry and Biomedical Sciences and Department of Medicine

Michael G. DeGroote School of Medicine

McMaster University

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/brands/atcc

Creating Synthetic Protein-Protein Interaction Networks and Implications for Endogenous Network Discovery

Cells utilize networks that span both temporal and spatial organizations, encompassing many individual steps of regulation. While the regulatory regimes to build networks in synthetic biology has grown from solely transcription to also include protein or RNA modalities, circuits comprised solely of protein-protein interactions have yet to be produced.

Speaker:

Deepak Mishra, Sc.D.

Postdoctoral fellow, MIT

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/brands/agilent-technologies-seahorse-bioscience/

Normalizing Your Seahorse XF Data with Automated Cell Counting

In this webinar we will discuss:

  • Why normalize Seahorse XF data at all?
  • Considerations for choosing a normalization method.
  • How automated cell counting can improve your data quality.
  • How to use Seahorse software tools to streamline data analysis.

Presented By:

Brian Dranka, Ph.D.

Biology Manager, Seahorse XF Products 

Agilent Technologies, Inc.

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/brands/agilent-technologies-seahorse-bioscience/

Stem Cell Metabolism Webinar

Recent work has emphasized the importance of metabolic states and energy balance in maintaining stem cell function. Studies at the interface of stem cell biology and metabolism have investigated how metabolic flux is linked to changes in cell fate and dysfunction and have highlighted potential strategies to restore or manipulate stem cell metabolism for therapeutic gains in aging and disease.

In this Cell Press webinar, Alessandro Prigione (MDC), Heather Christofk (UCLA), and Michael Teitell (UCLA) will discuss their work studying metabolic regulation of pluripotency and reprogramming, and provide insights into how cellular metabolism controls self-renewal, differentiation, and survival of human pluripotent stem cells

Presented by:

Alessandro Prigione

Independent Team Leader

Heather Christofk

Associate Professor, Biological Chemistry, Molecular & Medical Pharmacology

Michael Teitell

Professor, Latta Endowed Chair and Chief of the Division of Pediatric and Neonatal Pathology

Jon Saxe

Scientific Editor, Cell Stem Cell

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/brands/agilent-technologies-seahorse-bioscience/

Quantifying Cellular ATP Production Rate Using Agilent Seahorse XF Technology

  • How the new Seahorse XF Real-Time ATP Production Rate Assay quantifies ATP production from Oxygen Consumption Rate (OCR) and Extracellular Acidification Rates (ECAR) measurements.
  • Why ATP Production Rate is more informative than end-point total ATP level assays to provide information about cellular activities and energy demand.
  • How to apply the assay to quantify cellular metabolic phenotypes, detect and quantify metabolic switching and uncover pathway or fuel liabilities.

Speaker:

David Ferrick, PhD

Senior Director Marketing

Cell Analysis Division, Agilent

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