Studying Apoptosis in Cancer - Level up with Promega
Promega Products are distributed only in New Zealand by In Vitro Technologies
Apoptosis in Cancer
Investigating apoptosis is essential for understanding cancer biology and advancing cancer therapeutics. See the bigger picture in your cancer research by measuring apoptosis in real time using Promega’s range of simple sensitive fluorescent and luminescent assays. Sensitive and simple assays for publication-ready results.
THE APOPTOSIS IN CANCER RANGE |
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RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay |
PMJA1011 (100 assays), PMJA1012 (1000 assays) |
| ApoTox-Glo™ Triplex Assay | PMG6320 (10mL), PMG6321 (5 x 10mL) |
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Caspase-Glo® 3/7 Assay System |
PMG8090 (2.5mL), PMG8091 (10mL) , PMG8092 (100mL), PMG8093 (10 x 10mL) |
Want to incorporate real-time assays into your research to get the full picture?
> Watch the webinar
Introduction to Apoptosis
Promega RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay
The RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay measures the real-time exposure of phosphatidylserine (PS) on the outer leaflet of cell membranes during the apoptotic process.
The reagent contains Annexin V luciferase fusion proteins that bind to PS during early apoptosis and are detected with a simple luminescence signal. A DNA-binding dye, enters the cell and generates a fluorescent signal upon loss of membrane integrity.
How the assay works
See how the Realtime Glo Annexin V and Apoptosis assay was used in the development of liposome -based treatments towards lung cancer
> Read the Article
Promega ApoTox-Glo™ Triplex Assay
Measure Viability, Cytotoxicity, and Apoptosis in the same sample well using one assay containing:
- A fluorescent marker to measure cell viability
- A second fluorescent marker to measure loss of membrane integrity simultaneously
- A luminescent marker to monitor apoptosis
See how ApoTox-Glo was used to assess nanoparticle therapeutics against ovarian cancer
> Read the Article
Promega Caspase-Glo® 3/7 Assay System
The Caspase-Glo® 3/7 assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimized for caspase-3/7 activity, luciferase activity and cell analysis.
The stabilized luciferase and proprietary buffer system improve assay performance across a wide range of assay conditions, and the assay is less likely to be affected by compound interference than fluorescent- or colorimetric-based assays. The caspase activity assay is designed for use with multiwell plate formats using either purified enzyme or cells in culture and can be multiplexed with other cell-based assays. High sensitivity allows use of the Caspase-Glo® 3/7 assay in 3D cell culture systems.
