Questions? Feedback? powered by Olark live chat software
All Site

ATCC image 14T1-Luc2
For in vivo bioluminescence imaging of xenograft animal model for human cancer research and in cell-based assays for cancer research.

ATCC image 2C6/IacZ7
From rattus norvegicus this is a subclone of the C6/IacZ cell line. One of few models that permit quantitative analysis of microscopic tumor in the brain.

ATCC image 3
KRAS mutant-A375 Isogenic-LUC2
BRAF drug resistant melanoma model. Luciferase expression makes this cell line ideal for in vivo bioluminescence imaging of xenograft animal model for cancer research.

CAR-T cell-based therapeutics have emerged as a promising immunotherapy for treating specific leukemias, lymphomas, and myelomas. In this exciting approach to treating refractory cancers, T cells are isolated from a patient’s blood via apheresis. The cells are then incubated with the cytokine interleukin 2 and anti-CD3 antibodies to stimulate proliferation. Ex vivo silencing of genes involved in graft rejection is conducted to aid in improving T-cell stability after infusion. Once expanded, the appropriate CAR is introduced into the cells by retroviral transduction. These effector CAR-T cells are then infused back into the patient where they can exert their cytotoxic effects on tumor cells.

To provide target cells for immuno-oncology researchers adopting BLI assays, we generated CAR-T Target Luciferase Reporter Cells that can be used to examine the function of CAR-T cells. These reporter cells naturally express high levels of clinically relevant CAR-T target antigens on the cell surface. ATCC’s CAR-T Target Luciferase Reporter Cells were derived from a variety of highly malignant liquid and solid cancer types, namely B cell lymphoma, Burkitt’s lymphoma, Non-Hodgkin’s B cell lymphoma, and ductal breast carcinoma. These novel target cells were generated from parental tumor cells that have high endogenous expression of target antigens such as CD19, CD20, and HER2. Stable luciferase-expressing clones were engineered to display high signal-to-noise ratios, aiding in data interpretation.

CGT Main HP V2 banner