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Seahorse XF Cell Mito Stress Test Kit

SEA103015100 1SEA103015100 2

Functional mitochondrial measurements are key to understanding cellular activation, proliferation, differentiation and dysfunction. The Seahorse XF Cell Mito Stress Test provides a complete mitochondrial profile, revealing critical information not evident in basal metabolism measurements alone. 


  • Provides a robust test for analyzing the key parameters of mitochondrial function
  • Measure: Basal respiration, ATP-linked respiration, H+ (Proton) Leak, Maximal Respiration, Spare Respiratory Capacity, and Non-mitochondrial respiration.
  • Kit provides quality-controlled, pre-measured reagents and a standard method for conducting a Seahorse XF Cell Mito Stress Test
  • Seahorse XF Report Generator automatically calculates the key parameters of your samples, simplifying data analysis
  • Seahorse XF Cell Mito Stress Test Report Generator
  • Let’s talk about Mitochondrial Respiration
  • For Research Use Only. Not for use in diagnostic procedures.


Six single-use packets, each packet contains one vial of each:
Oligomycin, FCCP, and rotenone/antimycin A.
Number of Assays: Six Seahorse XF96/XF24 microplate assays
Shipping Conditions: Ships at ambient temperature
Storage Requirements: Store at room temperature


Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF/XFe Analyzer in an Hypoxic Environment

Measuring Mitochondrial Respiration in Intact Skeletal Muscle Fibers with an Agilent Seahorse XF24/XFe24 Analyzer 

This Application Note describes a method for profiling mitochondrial function in cells responding to stress.