Sommer A, Kordowski F, Buch J et al. Phosphatidylserine exposure is required for ADAM17 sheddase function. Nat Commun. May 10 2016 12:00AM [PMID:27161080] (FLOW, Human) |
FLOW |
Human |
Lew ED, Oh J, Burrola PG et al. Differential TAM receptor-ligand-phospholipid interactions delimit differential TAM bioactivities.Details:Polarity Sensitive Indicator of Viability Flow Assay Kit used for quantifying apoptosis in adherent cells or supernatant cells from Axl_TAM TKO MEF cultures - cells stained with propidium iodide which bind dead cells and pSIVA which fluoresces when bound to PtdSer (data in Figure 4B). Elife. 2014 Oct 24 [PMID:25265470] (FLOW, Mouse) |
FLOW |
Mouse |
Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells.Details:Fig 4: mouse splenocytes, EL4 mouse lymphoma cells, human PBMC, Jurkat human T cells; Fig 5: Jurkat. Cell Biol. Int. 2014 May 07 [PMID:24804954] (Flow-CS, Human, Mouse) |
Flow-CS |
Human, Mouse |
Nagaria TS, Williams JL, Leduc C et al. Flavopiridol synergizes with sorafenib to induce cytotoxicity and potentiate antitumorigenic activity in EGFR/HER-2 and mutant RAS/RAF breast cancer model systems.Details:Flow cytometry (Cell surface): MDA-MB-231 (Fig 4A) and MDA-MB-468 (Fig 4B) adenocarcinoma cells. Neoplasia. 2013 Aug [PMID:23908594] (Flow-CS) |
Flow-CS |
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Suhane S, Kanzaki H, Arumugaswami V et al. Mitochondrial NDUFS3 regulates the ROS-mediated onset of metabolic switch in transformed cells.Details:pSIVA-IANBD Flow Kit: Flow (Cell Surface): Fig 1 (HEK293 cells). pSIVA-IANBD was used to determine the basal level of apoptosis in HEK cells. Biol Open. 2013 Mar 15 [PMID:23519235] (Flow-CS) |
Flow-CS |
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Demchenko AP. The change of cellular membranes on apoptosis: fluorescence detection.Details:Live imaging: pSIVA as an important advancement in annexin based methodology. Exp Oncol. 2012 Oct [PMID:23070011] |
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Ruggiero L, Connor MP, Chen J et al. Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina.Details:Live tissue imaging (mouse retina): Figs 4, 5. S4. pSIVA-IANBD was added to dissected live mouse retina and shown to label the tips of photoreceptor outer segments (POS). The results suggested that phosphatidylserine (PS) exposure is specific to the POS surface. Furthermore, enhanced PS exposure preceded rod shedding and phagocytosis, suggesting that surface PS exposure promotes these processes. Proc Natl Acad Sci U S A. 2012 May 22 [PMID:22566632] (Mouse) |
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Mouse |
Krajewska M, You Z, Rong J et al. Neuronal deletion of caspase 8 protects against brain injury in mouse models of controlled cortical impact and kainic acid-induced excitotoxicity.Details:IF (mouse primary neuron cultures): Figs 2B, 3. Cells were labeled with pSIVA-IANBD for 15 min, fixed and stained with a MAP2 antibody and DAPI. Cells double-stained with both pSIVA-IANBD and MAP2 (neuronal marker) were identified as degenerating neurons. PLoS One. 2011 [PMID:21957448] (Mouse) |
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Mouse |
Graewe S, Rankin KE, Lehmann C et al. Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress.Details:IF (HepG2 cells infected with P. berghei parasites), Fig 1 PLoS Pathog. 2011 Sep [PMID:21909271] (ICC/IF) |
ICC/IF |
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Warnes G, Martins S. Real-time flow cytometry for the kinetic analysis of oncosis.Details:Live imaging: pSIVA as an assay for real-time detection of apoptosis. Cytometry A. 2011 Mar [PMID:21254392] |
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Zhang QC, Yeh TL, Leyva A et al. A compact beta model of huntingtin toxicity.Details:A pSIVA-IANBD based cell suspension toxicity assay was used to determine cell viability in mouse Neuro2A (neuroblastoma) overexpressing huntingtin proteins (Fig 4). J Biol Chem. 2011 Mar 11 [PMID:21209075] (Mouse) |
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Mouse |
Skommer J, Darzynkiewicz Z, Wlodkowic D. Cell death goes LIVE: technological advances in real-time tracking of cell death.Details:Live cell imaging (etoposide treated cell lines & NGF-deprived primary neuronal cultures): Discussion about tools for tracking cell death real-time. Cell Cycle. 2010 Jun 15 [PMID:20519963] |
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Kim YE, Chen J, Chan JR et al. Engineering a polarity-sensitive biosensor for time-lapse imaging of apoptotic processes and degeneration.Details:Real-time live-cell imaging and time-lapse microscopy of apoptosis: Fig 2 (Cos-7 cells), Fig 3 (neuronal degeneration), Fig 4 (axonal degeneration), Fig 5 (rescue of neuronal degeneration as visualized by pSIVA). Nat Methods. 2010 Jan [PMID:19966809] |
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