Intracellular Toll-like Receptor Staining Flow Cytometry Kit Summary
| Kit Type |
Flow Cytometry Kit |
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
Applications/Dilutions
| Application Notes |
Intracellular TLR staining flow kit is optimized for intracellular staining of cells in flow cytometric or immunocytochemical applications. It is designed and optimized to minimize non-specific staining while maximizing signal-to-noise ratio for clear and consistent data. |
| Publications |
| Read Publications using NBP2-26248 in the following applications: |
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Alternate Names for Intracellular Toll-like Receptor Staining Flow Cytometry Kit
- Intracellular TLR Staining
- Intracellular Toll like Receptor Staining
- Permeabilization buffer
Background
Fixation buffer is used to "fix" the cells/cellular proteins prior to permeabilization. Permeabilization buffer permeabilizes the cell membrane, allowing the detecting antibodies access to intracellular proteins. It is supplied as a 10X solution and can be diluted with deionized water to its final 1X working concentration.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Publications for Intracellular Toll-like Receptor Staining Kit (NBP2-26248)(3)
We have publications tested in 2 confirmed species: Human, Canine.We have publications tested in 2 applications: FLOW, Flow-IC.
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FLOW (1)
Flow-IC (2)
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Showing Publications 1 -
3 of 3.
| Publications using NBP2-26248 |
Applications |
Species |
|---|
| Nasr AS, Fawzy SM, Gheita TA, El-Khateeb E. Nasr AS, Fawzy SM, Gheita TA, El-Khateeb E. Expression of Toll-like receptors 3 and 9 in Egyptian systemic lupus erythematosus patients. Z Rheumatol. 2015 Dec 17 [PMID: 26680366] (FLOW) Z Rheumatol. 2015 Dec 17 [PMID:26680366] (FLOW) |
FLOW |
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| Burgener IA, Jungi TW. Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008 Jul 15 [PMID: 18439687] (Flow-IC, Canine)Details:Products cited for canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit 2. IMG-416A (TLR2), Flow (Cell Surface), Fig 1 and Tables 1 & 2 3. IMG-315A (TLR3), Flow (Intracellular), Tables 1 & 2 4. IMG-417A (TLR4), Flow Vet Immunol Immunopathol. 2008 Jul 15 [PMID:18439687] (Flow-IC, Canine) |
Flow-IC |
Canine |
| Klonowska-Szymczyk A, Wolska A, Robak T. Klonowska-Szymczyk A, Wolska A, Robak T. Expression of Toll-Like Receptors 3, 7, and 9 in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus. Mediators of Inflammation. 2014 Feb 25 (Flow-IC, Human)Details:PBMC: Figs 1-4 &Tables 1,2. Mediators of Inflammation. 2014 Feb 25 (Flow-IC, Human) |
Flow-IC |
Human |
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Flow Cytometry
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Additional Products
Intracellular Toll-like Receptor Staining NBP2-26248
- Intracellular Toll-like Receptor Staining Kit
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