Permits and Restrictions |
View Permits |
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Organism | Homo sapiens, human |
Tissue | skin |
Morphology | Spindle-shaped; cells are bipolar and refractile. |
Growth Properties | Adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Age | Adult |
Gender | Lot-specific |
Ethnicity | Lot-specific |
Applications | Response to pathogens, skin aging, wound healing, gene delivery, skin diseases (e.g., scleroderma); cosmetics research/testing. |
Product Format | frozen 1 mL |
Storage Conditions | -130°C or below |
Comments | Serum-free medium supports excellent growth curves and normal morphology, as well as serum-free (not animal-free) experimental conditions. The presence of 2% fetal bovine serum in the Fibroblast Growth Kit-Low serum supports more prolific growth compared to the serum-free medium. |
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Complete Growth Medium |
Table 1. If using the Fibroblast Growth Kit–Serum-Free (ATCC® PCS-201-040), add the indicated volume for each component in the order shown.
Table 2. If using the Fibroblast Growth Kit–Low Serum (ATCC® PCS-201-041), add the indicated volume for each of the following components:
Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of either of the optional components (GA solution or PSA solution) to be added to the complete growth media is summarized in Table 3.
Table 3. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)
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Subculturing |
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Volume | 1 mL |
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Cells per Vial | One vial contains a minimum of 5 x 105 viable cells. |
Sterility Tests | Bacteria and Yeasts: Negative. Mycoplasma: Negative |
Viral Testing | Hepatitis B: Negative Hepatitis C: Negative HIV: Negative |
Viability | ≥ 70% when thawed from cryopreservation |
Population Doubling Time | ≥ 10 doublings in serum-free media |
C of A | Certificate of Analysis |
Permits |
These permits may be required for shipping this product to Australia:
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Basic Documentation | Product Sheet Certificate of Analysis SDS |
References |
Rakowska P, Lamarre B, Ryadnov M. Probing label-free intracellular quantification of free peptide by MALDI-ToF mass spectrometry. Methods 68(2):331-37, 2014. PubMed: 24657280 Malpass G, Arimilli S, Prasad G, Howlett C. Regulation of Gene Expression by Tobacco Product Preparations in Cultured Human Dermal Fibroblasts. Toxicol Appl Pharmacol 279(2): 211-219, 2014. PubMed: 24927667 Malpass G, Arimilli S, Prasad G, Howlett A. Complete artificial saliva alters expression of proinflammatory cytokines in human dermal fibroblasts. Toxicol Sci 134(1):18-25, 2013. PubMed: 23629517 Kim S, Moon S, Lee Y, et al. Alternative xeno-free biomaterials derived from human umbilical cord for the self-renewal ex-vivo expansion of mesenchymal stem cells. Stem Cells Dev 22(22):3025-38, 2013. PubMed: 23786292 Whiteley J, Bielecki R, Li M, et al. An expanded population of CD34+ cells from frozen banked umbilical cord blood demonstrate tissue repair mechanisms of mesenchymal stromal cells and circulating angiogenic cells in an ischemic hind limb model. Stem Cell Rev 10(3):338-50, 2014. PubMed: 24443055 Pandit V, Zuidema J, Venuto K, et al. Evaluation of multifunctional polysaccharide hydrogels with varying stiffness for bone tissue engineering. Tissue Eng Part A 19(21-22):2452-63, 2013. PubMed: 23724786 Golberg A, Bei M, Sheridan R, Yarmush M. Regeneration and control of human fibroblast cell density by intermittently delivered pulsed electric fields. Biotechnol Bioeng 110(6):1759-68, 2013. PubMed: 23297079 Ansari C, Tikhomirov G, Hong S, et al. Development of novel tumor‐targeted theranostic nanoparticles activated by membrane‐type matrix metalloproteinases for combined cancer magnetic resonance imaging and therapy. Small 10(3):566-75, 2014. PubMed: 24038954 Pusztai E, Toulokhonova I, Temple N, et al. Synthesis and photophysical properties of asymmetric substituted silafluorenes. Organometallics 32(9):2529-35, 2013. Cam M, Bid H, Xiao L, et al. p53/TAp63 and AKT regulate mTORC1 signaling through two independent parallel pathways in the presence of DNA damage. J Biol Chem 289(7):4083-94, 2013. PubMed: 24366874 |