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Separation and purification of large biomolecules

The Tantti® Monolith hyperporous polymer is specifically designed for downstream separation and purification of large biomolecules – particularly in the production of biologics (e.g. protein drugs, vaccines, antibodies etc.).

Tantti® Monoliths are now available as DuloCore™ pre-packed columns. The key differentiating characteristic, in comparison to traditional purification columns, is its highly connected macropore structure that confers superior porosity, which is ideal for purifying large biomolecules at high loads:

  • Minimise the shear stress from Eddy currents affecting yield of biomolecules
  • Enables higher flow rates
  • Lower back pressure

The figure below depicts purification of virus particles using a Tantti® column. The interconnected macropores allow samples to pass through the column easily, while capturing impurities.

 

For more information, download the Application Notes below.

Capturing H7N9 Viral Particles

2705 Tantti App Note1

EV-71 purification

2705 Tantti App Note2

Separation of AAV8 Viral Capsid

2705 Tantti App Note3

Tantti® bioscaffolds for 3D cell culture

Tantti® BioScaffolds platform technology creates highly ordered micro-porous 3D BioScaffold and UniTantrix™ Microcarrier, which can be prepared with a variety of biological materials (such as collagen or animal-free materials).

Tantti® SpherTantrix is a Degradable 3D Cell Culture Scaffold

 

  Tantti® 3D Cell Culture scaffold has the following features:

  • manufactured from medical grade gelatin with excellent mechanical properties and thermal stability. 
  • The unique 3D Cell Culture scaffold with micrometer pore size, more surface area and highly connectivity is designed by the pioneer microfluid perforating technology licensed by Academia Sinica exclusively.
  • The special porosity structure allows fully gas exchange and nutrient metabolism upregulating the cell viability and proliferation.
  • Importantly, Tantti® 3D Cell Culture scaffold is dissolvable by using a specific enzyme and the cell recovery rate reaches to 100%.
  • Pore size, thickness and mechanical properties are adjustable to meet customer needs.

Advantages of Tantti® SpherTantrix, with its unique 3D porous structure:

  • Fast spheroids formation: ready for drug screening after only 2-3 days culture
  • Uniform spheroids: reliable data for potency prediction
  • Consistency: reproducible results with minimal intra- and inter-variations between well plates
  • Ready to use: compatible with most commercial high throughput screening systems
  • Easy inspection: direct observation of spheroids under an optical microscope

Applications of 3D bio-scaffold in biomedical science

The 3D bio-scaffold plays an important role in tissue engineering, oncology, regenerative medicine, drug screening and stem cell biology study.

  • To engineer an artificial organ, cells express similar behaviors, such as cell proliferation, ECM production, cell-cell interaction and cell-ECM interaction with those are in vivo through a 3D bio-scaffold.
  • For drug screening, to avoid the efficacy-prediction error and side effects, an appropriate model is necessary for drug evaluation.
  • Furthermore, the 3D scaffolds as an in vitro model are also used for drug evaluation and long-term clinical effects. 

UniTantrix™Microcarrier

What is UniTantrix™ Microcarrier?

  • The UniTantrix microcarrier provides a 3D construct for anchorage-dependent cells attachment and proliferation. It is recommended that 3D microcarrier culture is supporting the option of replacing 2D monolayer culture for cell expansion.
  • Microcarriers are made of either natural materials, such as gelatin, collagen and cellulose or synthetic materials, such as polystyrene(PS), polyhydroxyethylmethacrylate(pHEMA) and DEAE-dextran. Natural materials or its derivates with better biocompatibility, softer mechanical properties and higher reproducibility variation than synthetic materials.
  • In addition, lacking binding sites or functional groups of synthetic materials for cell adhesion is one of the major issues for cell expansion. Therefore, natural materials-based microcarrier has recently become more popular.

Features of UniTrantrix™

  • UniTantrix™ microcarrier is a unique macroporous construct with highly connectivity uniform pores and numerous surface area (6000 cm2/g).
  • It is manufactured by medical-grade gelatin providing flexible mechanical properties and stability.
  • The highly connective and uniform pores allow cells to adhere, proliferate, migrate and differentiate and the nutrients, oxygens and metabolic waste exchange effectively from the external to the internal microcarrier.
  • The macroporous microcarrier as a house-like structure to cells for protecting cells from shear stress exposure.

In addition, the microcarrier is dissolved easily by specific enzymes and the cell recovery rate is almost 100%.

UniTantrix™ Microcarrier Applications in Bio-Medical Science

  • Cell number expansion is the first priority in tissue engineering, cell therapy and vaccine manufacturing. Although the 2D cell culture is easy to apply for cell expansion, the issues such as time consuming, labor intensive and the unexpectable cell behaviors need to be defined.
  • Therefore, microcarriers which provide a 3D culture platform and greater surface area for cell adhesion and proliferation, are able to reach the ideal cell number for tissue engineering, cell therapy and vaccine manufacturing.

Tantti® Monolith

Tantti® Monolith has a unique global patent, we call it IMP method (In-Mold Polymerization) and we use In-Mold Polymerization to produce a highly connected honeycomb structure for the production of biological products and other related products. The process is expected to greatly break through the bottleneck of purification for macro molecules, especially accelerate the production of various biologic products such as protein, antibody analysis, exosome and viral particle purification, thereby effectively reducing the cost of protein drugs and vaccine production. In addition, this IMP technology can be used to adsorb and remove various trace substances, such as endotoxin, circulating tumor DNA, DNA impurities in the vaccine production process, host cell protein HCP, etc., for industrial application. From the laboratory research to the application of downstream purification and mass production process, Tantti’s IMP technology is a very pioneering idea and create excellent product value for downstream purification materials. We believe the monolith of Tantti can provide new solution for unmet need in the analysis and purification market.

Contact your Territory Manager to request your FREE sample.

Email: cher-lynn.soh@invitro.com.au

 

 

Tantti Monolith