Hi, my name is Milo – here to unlock your single cell proteome.
Milo™ is the world's first automated single-cell western (scWestern) platform. He does Single-Cell Westerns that’ll let you measure protein expression in thousands of single cells in a single run. You’ll get it all done in 4–6 hours with no overnight transfer step. And you can use off-the-shelf primary Western antibodies too. Milo measures protein expression heterogeneity and identifies cell subpopulations so you can make measurements you can’t make any other way and uncover novel insights about your samples!
One simple workflow
Add 1 mL of a single-cell suspension onto an scWest chip. Individual cells settle into microwells patterned into the pre-cast polyacrylamide gel. Milo lyses the cells, does a rapid (~1 min) SDS-PAGE separation on each single-cell lysate and immobilizes the proteins in the gel. Probe with conventional Western primary and fluorescent secondary antibodies in the probing chamber and image chip fluorescence. Scout Software analyses images to extract peak area for each target detected in each single-cell lysate.
One simple process lets you measure diverse targets and profile heterogeneity in your complex samples!
Milo performs Single-Cell Westerns to measure protein expression in thousands of single cells in a single run. You’ll get it all done in 4–6 hours with no overnight transfer step. And you can use off-the-shelf primary Western antibodies too — try that with flow cytometry! Milo measures protein expression heterogeneity and identifies cell subpopulations so you can make measurements you can’t make any other way and uncover novel insights about your samples.
You can use Milo to:
• Validate single-cell RNA data at the protein level
• Measure proteins that don’t have good flow antibodies
• Simplify challenging flow assays (e.g., transcription factors)
• Measure protein isoform heterogeneity
• Analyse samples with low cell numbers (e.g., FACS-sorted cells or rare stem cells)
• Quantify transfection efficiency
• Reveal heterogeneity obscured by bulk Western
What can Milo do to help you?
Download the Multiplexing to theInterested in other protein characterisation technologies? See what else Protein Simple has to offer!
Want more single cell solutions? Meet our single cell dispenser, Pala.
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