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Single Cell Dispensers from Namocell make single cell dispensing and sorting fast and easy

These benchtop instruments use proprietary microfluidics technology that combines flow cytometry and liquid dispensing to sort and dispense single cells directly into 96-well or 384-well plates. It simplifies and empowers a number of single cell applications, including cell line development and engineering, single cell genomics, iPSC and CRISPR cloning, antibody discovery, synthetic biology, and rare cell isolation such as circulating tumour cells (CTCs) and circulating foetal cells.

Your single cell isolation – sorted!

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Namocell Applications

Scientists have traditionally identified antigen-specific monoclonal antibodies with two approaches, each with significant drawbacks that limit the efficiency of antibody discovery.

1.Screening for hybridoma clones. 
This entails isolating B cells and fusing them with immortalised myeloma cells. Limiting dilutions are often used to isolate the desired clones but is inherently limited by the Poisson distribution, making this approach highly labour-intensive and time-consuming.
2. Screening for antigen-specific immune cells.
Antigen-specific B cells can be directly isolated by FACS instruments, which sort cells at very high pressure (up to 70 psi), resulting in reduced cell viability. Additionally, these instruments are very difficult to operate and prone to clogging. 

In both workflows, Namocell’s Single Cell Dispensers can be used to quickly but gently isolate single cells for downstream analysis.

Namocell Protein Simple Workflow

For downstream analysis of monoclonal antibodies, utilise the technologies from Protein Simple to automate:

  • • ELISA
  • • Western blot
  • • Imaged capillary electrophoresis

BioTech Article 3B Cell SC App Note1Simple Western App Note1Open Access to Ella App Note2Accelerate App Note1

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Synthetic biology enables scientists to design new sequences of DNA from scratch to engineer novel biological functions and systems within microorganisms.

Identifying genetically engineered microorganisms can be challenging due to their scarcity in the larger population. The small size of these microorganisms can make isolating them even more challenging.

The workflow below is an example on how you can isolate the desired bacterial strains for outgrowth.

Microorganisms are transfected with the desired genetic information along with a fluorescent reporter gene, such as GFP. Individual GFP-positive cells are then identified and dispensed with the Namocell Pala Single Cell Dispenser, then cultured for downstream characterization.

Namocell Graph 1

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