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Pluripotent Stem Cell Transcription Factor Antibody Pack Summary

Immunogen NB100-58842: A synthetic peptide made to the mouse Nanog protein (within residues 1-50). [Swiss-Prot Q80Z64]. NB100-2379-A synthetic peptide made to an internal portion
Specificity NB100-58842 is specific for mouse Nanog protein. NB100-2379-OCT4 - Embryonic Stem Cell Marker NB110-37235 - SOX2 - Embryonic Stem Cell Marker
Kit Type Kit
Clonality Polyclonal
Host Rabbit
Purity Immunogen affinity purified

Applications/Dilutions

Application Notes This antibody is useful for: NB100-37235 Western blot analysis where a band at ~40 kDa is be seen, representing the SOX2 protein. Has also been used in immunohistochemical and immunocytochemical applications. NB100-58842- Western blot analysis where a band at ~40 kDa is be seen, representing the SOX2 protein. Has also been used in immunohistochemical and immunocytochemical applications. NB100-2379-This antibody is useful for Western blot, where a band is seen at ~40 kDa. It is also useful for immunocytochemistry/immunofluorescence and flow cytometry.
Reviewed Applications
Read 1 Review rated 5 using NBP1-42823 in the following application:
  • Western Blot
Publications
Read Publication using NBP1-42823 in the following applications:
  • WB 1 publication

Reactivity Notes

NB100-58842-Mouse and Human. Reactivity with human Nanog is unlikely owing to divergence of sequence between this ortholog and mouse Nanog at the site to which the epitope maps. NB100-2379-Reacts with human, monkey and mouse. 93% sequence identity with cow and pig proteins NB110-37235 - Human, sheep and mouse. 92% sequence identity with chicken, 86% sequence identity with Xenopus, and 71% sequence identity with Zebrafish proteins.

Packaging, Storage & Formulations

Storage Store at 4C. Do not freeze.
Preservative 0.05% Sodium Azide
Purity Immunogen affinity purified

Kit Components

Components
  1. NB100-58842 (Nanog Antibody)
  2. Pluripotent Stem Cell Transcription Factor Antibody Pack (OCT4 Antibody)
  3. Pluripotent Stem Cell Transcription Factor Antibody Pack (SOX2 Antibody)

Notes

Kit contains full sized units of the following: NB100-2379, NB110-37235, and NB100-58842.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Publications for Pluripotent Stem Cell Transcription Factor Antibody Pack (NBP1-42823)(1)

We have publications tested in 1 confirmed species: Human.We have publications tested in 1 application: WB. Submit a Publication Filter By Application WB (1) All Applications Filter By Species Human (1) All Species Showing Publication 1 - 1 of 1.
Publication using NBP1-42823 Applications Species
Zhang C, Zhi WI, Lu H et al. Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells. Oncotarget. 2016 Aug 31 [PMID: 27590511] (WB, Human) WB Human

Review for Pluripotent Stem Cell Transcription Factor Antibody Pack (NBP1-42823) (1) 51

Average Rating: 5 (Based on 1 review) We have 1 review tested in 1 species: Human. Submit a Review Reviews using NBP1-42823: Filter by Applications WB (1) All Applications Filter by Species Human (1) All Species
Images Ratings Applications Species Date Details
Enlarge reviewed by:Anonymous WB Human 09/02/2015 View

Summary

Application Western Blot
Sample Tested Human cancer cell whole cell lysate
Species Human

Blocking

Blocking Details Blocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio 1:1000, overnight

Secondary Antibody

Secondary Description Donkey anti-rabbit IgG, HRP
Secondary Manufacturer Cat# GE Healthcare, NA934V
Secondary Concentration 1:2000

Details

Detection Notes Regular Western blot Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
  • Western Blot
  • Flow Cytometry
  • Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
  • View all Protocols, Troubleshooting, Illustrated assays and Webinars.

Video Protocols

WB Video Protocol ICC/IF Video Protocol

FAQs for Pluripotent Stem Cell Transcription Factor Antibody Pack (NBP1-42823) (0)

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Additional Array Products

Array NBP1-42823
  • Pluripotent Stem Cell Transcription Factor Antibody Pack

Research Areas for Pluripotent Stem Cell Transcription Factor Antibody Pack (NBP1-42823)

Find related products by research area.
  • Stem Cell Markers

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