Pluripotent Stem Cell Transcription Factor Antibody Pack Summary
Immunogen |
NB100-58842: A synthetic peptide made to the mouse Nanog protein (within residues 1-50). [Swiss-Prot Q80Z64]. NB100-2379-A synthetic peptide made to an internal portion |
Specificity |
NB100-58842 is specific for mouse Nanog protein. NB100-2379-OCT4 - Embryonic Stem Cell Marker NB110-37235 - SOX2 - Embryonic Stem Cell Marker |
Kit Type |
Kit |
Clonality |
Polyclonal |
Host |
Rabbit |
Purity |
Immunogen affinity purified |
Applications/Dilutions
Application Notes |
This antibody is useful for: NB100-37235 Western blot analysis where a band at ~40 kDa is be seen, representing the SOX2 protein. Has also been used in immunohistochemical and immunocytochemical applications. NB100-58842- Western blot analysis where a band at ~40 kDa is be seen, representing the SOX2 protein. Has also been used in immunohistochemical and immunocytochemical applications. NB100-2379-This antibody is useful for Western blot, where a band is seen at ~40 kDa. It is also useful for immunocytochemistry/immunofluorescence and flow cytometry. |
Reviewed Applications |
Read 1 Review rated 5 using NBP1-42823 in the following application: |
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Publications |
Read Publication using NBP1-42823 in the following applications: |
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Reactivity Notes
NB100-58842-Mouse and Human. Reactivity with human Nanog is unlikely owing to divergence of sequence between this ortholog and mouse Nanog at the site to which the epitope maps. NB100-2379-Reacts with human, monkey and mouse. 93% sequence identity with cow and pig proteins NB110-37235 - Human, sheep and mouse. 92% sequence identity with chicken, 86% sequence identity with Xenopus, and 71% sequence identity with Zebrafish proteins.
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Preservative |
0.05% Sodium Azide |
Purity |
Immunogen affinity purified |
Kit Components
Components
|
- NB100-58842 (Nanog Antibody)
- Pluripotent Stem Cell Transcription Factor Antibody Pack (OCT4 Antibody)
- Pluripotent Stem Cell Transcription Factor Antibody Pack (SOX2 Antibody)
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Notes
Kit contains full sized units of the following: NB100-2379, NB110-37235, and NB100-58842.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
Publications for Pluripotent Stem Cell Transcription Factor Antibody Pack (NBP1-42823)(1)
We have publications tested in 1 confirmed species: Human.We have publications tested in 1 application: WB.
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Publication using NBP1-42823 |
Applications |
Species |
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Zhang C, Zhi WI, Lu H et al. Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells. Oncotarget. 2016 Aug 31 [PMID: 27590511] (WB, Human) |
WB |
Human |
Review for Pluripotent Stem Cell Transcription Factor Antibody Pack (NBP1-42823) (1)
51
Average Rating: 5 (Based on 1 review)
We have 1 review tested in 1 species: Human.
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Reviews using NBP1-42823:
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WB (1)
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Human (1)
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Images |
Ratings |
Applications |
Species |
Date |
Details |
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Enlarge |
reviewed by:Anonymous
|
WB |
Human |
09/02/2015 |
View
Summary
Application |
Western Blot |
Sample Tested |
Human cancer cell whole cell lysate |
Species |
Human |
Blocking
Blocking Details |
Blocking with 5% non-fat milk for 30 min |
Primary Anitbody
Dilution Ratio |
1:1000, overnight |
Secondary Antibody
Secondary Description |
Donkey anti-rabbit IgG, HRP |
Secondary Manufacturer Cat# |
GE Healthcare, NA934V |
Secondary Concentration |
1:2000 |
Details
Detection Notes |
Regular Western blot Protocol |
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Western Blot
- Flow Cytometry
- Immunoprecipitation
- Immunocytochemistry/Immunofluorescence
- Immunohistochemistry-Paraffin
- View all Protocols, Troubleshooting, Illustrated assays and Webinars.
Video Protocols
WB Video Protocol
ICC/IF Video Protocol
FAQs for Pluripotent Stem Cell Transcription Factor Antibody Pack (NBP1-42823) (0)
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Additional Array Products
Array NBP1-42823
- Pluripotent Stem Cell Transcription Factor Antibody Pack
Research Areas for Pluripotent Stem Cell Transcription Factor Antibody Pack (NBP1-42823)
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