TfR (Transferrin R) Antibody (OX26) Summary
| Immunogen |
PHA activated rat lymphocytes. |
| Marker |
Recycling Endosome Marker |
| Specificity |
Labels dividing cells of various types but also binds to a number of non-dividing normal tissues and endothelium of brain capillaries. |
| Isotype |
IgG2a |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Gene |
TFRC |
| Purity |
Protein G purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.Learn about the Innovator's Reward
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Applications/Dilutions
| Dilutions |
- Western Blot 1:100-1:2000
- Flow Cytometry 1:50-1:200
- Immunocytochemistry/Immunofluorescence 1:10-1:500
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10-1:500
- Immunohistochemistry-Paraffin 1:10-1:500
- CyTOF-ready
- Flow (Intracellular)
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| Application Notes |
Use in IHC was reported in the scientific literature (PMID: 11818402). |
| Publications |
| Read Publications using NB200-585 in the following applications: |
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Reactivity Notes
Rat.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
PBS |
| Preservative |
0.05% Sodium Azide |
| Concentration |
1.0 mg/ml |
| Purity |
Protein G purified |
Alternate Names for TfR (Transferrin R) Antibody (OX26)
- CD71 antigen
- CD71
- CD71TRFR
- OX26 monoclonal antibody
- OX26 transferrin receptor
- p90
- T9
- TfR (Transferrin R)
- TfR (TransferrinR)
- TfR
- TFR1
- TFRC
- TR
- transferrin receptor (p90, CD71)
- transferrin receptor protein 1
- TRFR
Background
OX26 TfR clone is useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. In comes in a variety of pre-conjugated fluorophores for histochemical and flow applications.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for TfR (Transferrin R) Antibody (NB200-585)(14)
We have publications tested in 1 confirmed species: Rat.We have publications tested in 2 applications: FLOW, IHC-Fr.
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FLOW (3)
IHC-Fr (1)
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Rat (4)
All Species
Showing Publications 1 -
10 of 14.
Show All 14 Publications.
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| Publications using NB200-585 |
Applications |
Species |
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| Couty JP, Rampon C, Leveque M et al. PECAM-1 engagement counteracts ICAM-1-induced signaling in brain vascular endothelial cells. J Neurochem. [PMID: 17662049] |
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| Gosk S, Vermehren C, Storm G et al. Targeting anti-transferrin receptor antibody (OX26) and OX26-conjugated liposomes to brain capillary endothelial cells using in situ perfusion. J Cereb Blood Flow Metab 24(11):1193-204. 2004 Nov. [PMID: 15545912] |
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| Cox JH, Lamb JR, Bal V et al. The phenotypic and molecular characterization of Nb2 lymphoma cells activated with IL-2 and human growth hormone. Immunology. 1989 Jan 1 [PMID: 15493267] (FLOW, Rat) |
FLOW |
Rat |
| Moos T, Morgan EH. Restricted transport of anti-transferrin receptor antibody (OX26) through the blood-brain barrier in the rat. J Neurochem 79(1):119-29. 2001 Oct. [PMID: 11595764] |
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| Crook K, Hunt SV. Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82. Dev Immunol. [PMID: 8924759] (FLOW, Rat) |
FLOW |
Rat |
| Broadwell RD, Baker-Cairns BJ, Friden PM et al. Transcytosis of protein through the mammalian cerebral epithelium and endothelium. III. Receptor-mediated transcytosis through the blood-brain barrier of blood-borne transferrin and antibody against the transferrin receptor. Exp Neurol. 1996 Nov 1 [PMID: 8912898] |
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| Skarlatos S, Yoshikawa T, Pardridge WM. Transport of [125I]transferrin through the rat blood-brain barrier. Brain Res. 1995 Jun 19 [PMID: 7552351] |
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| Paterson DJ, Williams AF. An intermediate cell in thymocyte differentiation that expresses CD8 but not CD4 antigen. J Exp Med. 1987 Nov 1 [PMID: 3500264] (FLOW, Rat) |
FLOW |
Rat |
| de Sousa M, Dynesius-Trentham R, Mota-Garcia F et al. Activation of rat synovium by iron. Arthritis Rheum. 1988 May 1 [PMID: 3377869] |
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| De Sousa M, Breedvelt F, Dynesius-Trentham R et al. Iron, iron-binding proteins and immune system cells. Ann N Y Acad Sci. 1988 June 1 [PMID: 3291685] |
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| Opstelten D, Deenen GJ, Rozing J, Hunt SV. B lymphocyte-associated antigens on terminal deoxynucleotidyl transferase-positive cells and pre-B cells in bone marrow of the rat. J Immunol. 1986 Jul 1 [PMID: 3086459] |
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| Hill JM, Ruff MR, Weber RJ, Pert CB. Transferrin receptors in rat brain: neuropeptide-like pattern and relationship to iron distribution. Proc Natl Acad Sci U S A. 1985 Jul 1 [PMID: 2989832] |
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| Perry VH, Gordon S. Modulation of CD4 antigen on macrophages and microglia in rat brain. J Exp Med. 1987 Oct 1 [PMID: 2443599] (IHC-Fr, Rat) |
IHC-Fr |
Rat |
| Chignola R, Colombatti M, Dell'Arciprete L et al. Distribution of endocytosed molecules to intracellular acidic environments correlates with immunotoxin activity. Int J Cancer. 1990 Dec 15 [PMID: 2123478] |
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Show All 14 Publications.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Western Blot
- Immunohistochemistry-Paraffin
- Immunocytochemistry/Immunofluorescence
- Flow Cytometry
- Immunohistochemistry-Frozen
- View all Protocols, Troubleshooting, Illustrated assays and Webinars.
Video Protocols
WB Video Protocol
ICC/IF Video Protocol
FAQs for TfR (Transferrin R) Antibody (NB200-585). (Showing 1 - 2 of 2 FAQs).
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A customer would like to use this antibody to perform an functional assay. The customer would like to know whether this antibody will block the binding of transferrin to the receptor. On the other hand, would you mind to provide the immunogenic sequence of this antibody?
- Unfortunately, this clone has not been mapped and the sequence of the epitope recognised is currently unknown. All that is currently known is that the clone binds an extracellular region not involved in transferrin binding.
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I was wondering if you have amino acid sequence information available for this monoclonal mouse anti-Rat TfR (Ox26) antibody.
- The immunogen used to create this product is PHA activated rat lymphocytes (SwissProt: Q99376). We have not mapped the epitope for the product.
Secondary Antibodies
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Isotype Controls
- Mouse Isotype Controls
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- View all of our Isotype Control Products
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Other Available Formats
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Alexa Fluor 405 Labeled
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NB200-585AF405
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Alexa Fluor 488 Labeled
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NB200-585AF488
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Alexa Fluor 647 Labeled
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NB200-585AF647
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Alexa Fluor 700 Labeled
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NB200-585AF700
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Allophycocyanin Labeled
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NB200-585APC
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DyLight 350 Labeled
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NB200-585UV
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DyLight 405 Labeled
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NB200-585V
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DyLight 488 Labeled
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NB200-585G
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DyLight 680 Labeled
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NB200-585FR
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DyLight 755 Labeled
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NB200-585IR
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PE Labeled
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NB200-585PE
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PerCP Labeled
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NB200-585PCP
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Learn about Custom Antibody Labeling
Additional TfR (Transferrin R) Antibody (OX26) Products
TfR (Transferrin R) NB200-585
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- TfR (Transferrin R) ELISA Kit
- TfR (Transferrin R) Lysates
- TfR (Transferrin R) Proteins
- TfR (Transferrin R) RNAi
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Bioinformatics Tool for TfR (Transferrin R) Antibody (NB200-585)
Discover related pathways, diseases and genes to TfR (Transferrin R) Antibody (NB200-585). Need help?
Read the Bioinformatics Tool Guide for instructions on using this tool.
Diseases for TfR (Transferrin R) Antibody (NB200-585)
Discover more about diseases related to TfR (Transferrin R) Antibody (NB200-585).
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Pathways for TfR (Transferrin R) Antibody (NB200-585)
View related products by pathway.
- Transport
- Localization
- Pathogenesis
- Reverse Transcription
- Cell Proliferation
- Endocytosis
- Translation
- Osteoclast Differentiation
- Aging
- Cell Cycle
- Cell Development
- Osteoclast Development
- Cell Activation
- Oxygen Homeostasis
- Stem Cell Differentiation
- Electron Transport
- Electron Transport Chain
- Brain Development
- Proteolysis
- Immune Response
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PTMs for TfR (Transferrin R) Antibody (NB200-585)
Learn more about PTMs related to TfR (Transferrin R) Antibody (NB200-585).
- Phosphorylation
- Acetylation
- Methylation
- Ubiquitination
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Research Areas for TfR (Transferrin R) Antibody (NB200-585)
Find related products by research area.
- Cell Biology
- Cellular Markers
- Hematopoietic Stem Cell Markers
- Immunology
- Signal Transduction
- Stem Cell Markers
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Blogs on TfR (Transferrin R).
Check out the latest blog posts on TfR (Transferrin R).
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Transferrin and the blood brain barrierTransferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post. |
