Novus CD133 Antibody, 0.1 mg Item ID: NOVNB12016518

CD133 Antibody Summary

Immunogen	Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865).
Marker	Stem Cell Marker
Specificity	CD133 - Hematopoietic Stem Cell Marker
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	PROM1
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase.Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	Western Blot 1:500-1:1000 Chromatin Immunoprecipitation ELISA 1:1000 Flow Cytometry 1:10-1:1000 Immunocytochemistry/Immunofluorescence 1:100 Immunohistochemistry 1:100-1:250 Immunohistochemistry-Frozen Immunohistochemistry-Paraffin 1:100-1:250
Application Notes	ELISA: 1/1000. WB: 1/1,000 - 1:2,000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29352176).
Publications	Read Publications using NB120-16518 in the following applications: ELISA 1 publication FLOW 1 publication Flow 1 publication ICC/IF 2 publications IHC 3 publications IHC-Fr 1 publication WB 1 publication

Reactivity Notes

Reacts with human, mouse and rat. porcine reactivity reported in scientific literature (PMID: 29352176).

Packaging, Storage & Formulations

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.02% Sodium Azide
Concentration	1.0 mg/ml
Purity	Immunogen affinity purified

Alternate Names for CD133 Antibody

• AC133 Stargardt disease 4 (autosomal dominant)
• AC133
• CD133 retinal 2
• CD133
• PROM1
• prominin (mouse)-like 1
• Prominin 1
• PROML1

Background

Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.