Novus Luciferase Antibody (LUC-Y), 0.05 mg Item ID: NOVNB10064800

Luciferase Antibody (LUC-Y) Summary

Immunogen	Recombinant luciferase expressed in E. coli.
Specificity	Detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase.Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	Western Blot 1:100 ELISA 1:100-1:2000 Flow Cytometry Immunohistochemistry 1:10-1:500 Immunohistochemistry-Frozen 1:10-1:500
Application Notes	For Flow Cytometry, membrane permeabilisation is required. For Flow Cytometry we recommend using 0.1ug per 1 x 10^6 cells.

Reactivity Notes

Reacts with Insect.

Packaging, Storage & Formulations

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	Phosphate buffered saline
Preservative	0.09% Sodium Azide
Concentration	1.0 mg/ml
Purity	Protein A purified

Alternate Names for Luciferase Antibody (LUC-Y)

• ec 1.13.12.7
• Fluc
• Luciferase
• luciferin 4 monooxygenase

Background

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.