Novus Lightning-Link HRP Antibody Labeling Kit, 5 Reactions (up to 4 mg) Item ID: NOV7010003

Publications for Lightning-Link HRP Kit (701-0003)(64)

We have publications tested in 6 applications: ELISA, FLOW, IA, IB, IP, WB. Submit a Publication Filter By Application ELISA (1) FLOW (1) IA (32) IB (4) IP (1) WB (5) All Applications Filter By Species All Species Showing Publications 1 - 10 of 64. Show All 64 Publications. Collapse Publications.

Publications using 701-0003	Applications	Species
Ghaffarian R, Roki N, Abouzeid A et al. Intra- and trans-cellular delivery of enzymes by direct conjugation with non-multivalent anti-ICAM molecules J Control Release 2016 Sep 28 [PMID:27473764] (WB)	WB
Laura RP, Dong D, Reynolds WF, Maki RA. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation PLoS One 2016 Feb 18 [PMID:26890638] (WB)	WB
Liao MC, Muratore CR, Gierahn TM et al. Single-Cell Detection of Secreted ABeta and sAPPa from Human IPSC-Derived Neurons and Astrocytes J. Neurosci. 2016 Feb 3 [PMID:26843653] (FLOW)	FLOW
Mir RA, Bele A, Mirza S et al. A novel interaction of ECD protein with R2TP complex component RUVBL1 is required for the functional role of ECD in cell cycle progression Mol Cell Biol 2015 Dec 28 [PMID:26711270] (WB)	WB
Ahn SB, Mohamedali A, Anand S et al. Characterisation of the interaction between heterodimeric alpha v beta 6 integrin and urokinase plasminogen activator receptor (uPAR) using functional proteomics. J Proteome Res 2014 [PMID:25318615]
Hu Z, Chang H, Ge M et al. Development of antigen capture ELISA for the quantification of EIAV p26 protein. Appl Microbiol Biotechnol. 2014 [PMID:25256618] (IA)	IA
Praekelt U, Reissbrodt R, Kresse A et al. Monoclonal antibodies against all known variants of EspA: development of a simple diagnostic test for enteropathogenic Escherichia coli based on a key virulence factor. J Med Microbiol 2014 [PMID:25231626] (IA)	IA
Debald M, Jin JP, Linke A et al. Calponin-h2: a potential serum marker for the early detection of human breast cancer?. Tumour Biol. 2014 [PMID:25099617] (IA)	IA
Saresella M, Piancone F, Marventano I et al. A role for the TIM-3/GAL-9/BAT3 pathway in determining the clinical phenotype of multiple sclerosis. FASEB J. 2014 [PMID:25091272]
Sun S, Henriksen K, Karsdal MA et al. Measurement of a MMP-2 degraded Titin fragment in serum reflects changes in muscle turnover induced by atrophy. Exp Ger 2014 [PMID:25077715] (IA)	IA
Kaever T, Meng X, Matho MH et al. Potent Neutralization of Vaccinia Virus by Divergent Murine Antibodies Targeting a Common Site of Vulnerability in L1 Protein. J Virol. 2014 [PMID:25031354]
Dong GZ, Lee JH, Ki SH et al. AMPK activation by isorhamnetin protects hepatocytes against oxidative stress and mitochondrial dysfunction. Eur J Pharmacol 2014 [PMID:24972246]
Sapkota M, Hottor TK, DeVasure JM et al. Protective Role of CYP2E1 Inhibitor Diallyl Disulfide (DADS) on Alcohol-Induced Malondialdehyde-Deoxyguanosine (M1dG) Adduct Formation. Alcohol Clin Exp Res. 2014 [PMID:24891074]
Sun S, Bay-Jensen AC, Karsdal MA et al. The active form of MMP-3 is a marker of synovial inflammation and cartilage turnover in inflammatory joint diseases. BMC Musculoskelet Disord. 2014 [PMID:24641725] (WB)	WB
Yau JW, Liao P, Fredenburgh JC et al. Selective depletion of factor XI or factor XII with antisense oligonucleotides attenuates catheter thrombosis in rabbits. Blood 2014 [PMID:24501216]
Dutta S, Dlugosz LS, Drew DR et al. Overcoming Antigenic Diversity by Enhancing theImmunogenicity of Conserved Epitopes on the MalariaVaccine Candidate Apical Membrane Antigen-1. PLoS Pathog. 2013 [PMID:24385910] (ELISA)	ELISA
Sand JM, Larsen L, Hogaboam C et al. MMP Mediated Degradation of Type IV Collagen Alpha 1 and Alpha 3 Chains Reflects Basement Membrane Remodeling in Experimental and Clinical Fibrosis - Validation of Two Novel Biomarker Assays. PLoS One 2013 [PMID:24376856]
Kim HK, Emolo C, Missiakas D, Schneewind O. A monoclonal antibody that recognizes the E domain of staphylococcal protein A. Vaccine 2013 [PMID:24291195] (IA)	IA
Murdock RC, Shen L, Griffin DK et al. Optimization of a Paper-Based ELISA for a Human Performance Biomarker. Anal Chem. 2013 [PMID:24206087] (IA)	IA
He X, Patfield S, Hnasko R et al. A Polyclonal Antibody Based Immunoassay Detects Seven Subtypes of Shiga Toxin 2 Produced by Escherichia coli in Human and Environmental Samples. PLoS One 2013 [PMID:24146860] (IA)	IA
Leeming DJ, Karsdal MA, Rasmussen LM et al. Association of Systemic Collagen Type IV Formation with Survival among Patients Undergoing Hemodialysis. PLoS One 2013 [PMID:23990924] (IA)	IA
Mellor L, Knudson CB, Hida D et al. Intracellular Domain Fragment of CD44 Alters CD44 Function in Chondrocytes. J Biol Chem 2013 [PMID:23884413]
Skjot-Arkil H, Clausen RE, Rasmussen LM et al. Acute Myocardial Infarction and Pulmonary Diseases Result in Two Different Degradation Profiles of Elastin as Quantified by Two Novel ELISAs. PLoS One 2013 [PMID:23805173] (IA)	IA
Nielsen MJ, Nedergaard AF, Sun S et al. The neo-epitope specific PRO-C3 ELISA measures true formation of type III collagen associated with liver and muscle parameters. Am J Transl Res 2013 [PMID:23634241] (IA)	IA
Sun S, Karsdal MA, Bay-Jensen AC et al. The development and characterization of an ELISA specifically detecting the active form of cathepsin K. Clin Biochem 2013 [PMID:23623829] (IA)	IA
Skinner C, McMahon S, Rasooly R et al. Purification and Characterization of Shiga Toxin 2f, an Immunologically Unrelated Subtype of Shiga Toxin 2. PLoS One 2013 [PMID:23555772] (WB)	WB
Iankov ID, Penheiter AR, Griesmann GE et al. Neutralization capacity of measles virus H protein specific IgG determines the balance between antibody-enhanced infectivity and protection in microglial cells. Virus Res 2012 [PMID:23266401]
Chambers AE, Griffin C, Naif SA et al. Quantitative ELISAs for serum soluble LHCGR and hCG-LHCGR complex: potential diagnostics in first trimester pregnancy screening for stillbirth, down's syndrome, preterm delivery and preeclampsia. Reprod Biol Endocrinol 2012 [PMID:23245345] (IA)	IA
Bayley R, Yang P, Buckley CD, Young SP. Measuring the specific activity of the protein tyrosine phosphatase Lyp. J Immunol Methods. 2012 [PMID:23219421] (IA)	IA
Vassiliadis E, Oliveira CP, Alvares-da-Silva MR et al. Circulating levels of citrullinated and MMP-degraded vimentin (VICM) in liver fibrosis related pathology Am J Transl Res 2012 [PMID:23145208] (IA)	IA
Walser P, Nicholas A, Howes M et al. Constitutive Formation of Caveolae in a Bacterium Cell 2012 [PMID:22901807]
Skot-Arkil H, Clausen R, Nguyen Q et al. Measurement of MMP-9 and -12 degraded elastin (ELM) provides unique information on lung tissue degradation BMC Pulmonary Medicine 2012 [PMID:22818364] (IA)	IA
Iankov ID, Penheiter AR, Carlson SK, Galanis E. Development of monoclonal antibody-based immunoassays for detection of Helicobacter pylori neutrophil-activating protein Journal of Immunological Methods 2012 [PMID:22750540] (IA)	IA
Ndonwi M, Girard TJ, Broze GJ. The carboxy-terminus of TFPIAlpha is required for its interaction with factor V and Va Journal of Thrombosis and Haemostasis 2012 [PMID:22738072]
Acker C, Forest S, Zinkowski R et al. Sensitive quantitative assays for tau and phospho-tau in transgenic mouse models Neurobiology of Aging 2012 [PMID:22727277] (IA)	IA
Zhang H, Guo X, Nelson E et al. Porcine Reproductive and Respiratory Syndrome Virus Activates the Transcription of Interferon alpha/beta (IFN-Alpha/Beta) in Monocyte-derived Dendritic Cells (Mo-DC) Veterinary Microbiology 2012 [PMID:22592217] (IA)	IA
Chan AH, Tan HC, Chow AY et al. A Human PrM Antibody That Recognizes a Novel Cryptic Epitope on Dengue E Glycoprotein PLoS One 2012 [PMID:22509258] (IA)	IA
Grise H, Frausto S, Logan T, Tang H. A Conserved Tandem Cyclophilin-Binding Site in Hepatitis C Virus Nonstructural Protein 5A Regulates Alisporivir Susceptibility Journal of Virology 2012 [PMID:22345441]
Goihl A, Rolle AM, Kahne T et al. Methodologic issues in the measurement of interleukin-16 in clinical blood samples using immunoassays Cytokine 2011 [PMID:22239948]
He J, Lai H, Brock C, Chen Q. A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants Journal of Biomedicine and Biotechnology 2012 [PMID:22187532] (IA)	IA
Leeming Dj, He Y, Veidal S et al. A novel marker for assessment of liver matrix remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M) Biomarkers 2011 [PMID:21988680] (IA)	IA
Veidal SS, Karsdal MA, Nawrocki A et al. Assessment of proteolytic degradation of the basement membrane: a fragment of type IV collagen as a biochemical marker for liver fibrosis Fibrogenesis & Tissue Repair 2011 [PMID:21970406] (IA)	IA
Veidal SS, Karsdal MA, Vassiliadis E et al. MMP Mediated Degradation of Type VI Collagen Is Highly Associated with Liver Fibrosis - Identification and Validation of a Novel Biochemical Marker Assay PLoS One 2011 [PMID:21935455] (IA)	IA
Hashimoto T, Yasuda S, Koide H et al. Aberrant splicing of the hRasGRP4 transcript and decreased levels of this signaling protein in the peripheral blood mononuclear cells in a subset of patients with rheumatoid arthritis PLoS One 2011 [PMID:21933395] (IB)	IB
Chan CE, Chan AH, Lim AP, Hanson BJ. Comparison of the efficiency of antibody selection from semi-synthetic scFv and non-immune Fab phage display libraries against protein targets for rapid development of diagnostic immunoassays Journal of Immunological Methods 2011 [PMID:21856306] (IA)	IA
Vu TT, Stafford AR, Leslie BA et al. Histidine-rich Glycoprotein Binds Fibrin(ogen) with High Affinity and Competes with Thrombin for Binding to the Gamma-Chain Journal of Biological Chemistry 2011 [PMID:21757718] (IA)	IA
Anderson N, Suliman I, Bandaletova T et al. Protein biomarkers in exfoliated cells collected from the human rectal mucosa: implications for colorectal disease detection and monitoring International Journal of Colorectal Disease 2011 [PMID:21698353] (IA)	IA
Zapater C, Chauvigne F, Norberg B et al. Dual Neofunctionalization of a Rapidly Evolving Aquaporin-1 Paralog Resulted in Constrained and Relaxed Traits Controlling Channel Function during Meiosis Resumption in Teleosts Mol Biol Evol 2011 [PMID:21653921] (IB)	IB
Vassiliadis E, Veidal SS, Simonsen H et al. Immunological detection of the type V collagen propeptide fragment, PVCP-1230, in connective tissue remodeling associated with liver fibrosis Biomarkers 2011 [PMID:21612338] (IA)	IA
Lemaire K, Moura RF, Granvik M et al. Ubiquitin Fold Modifier 1 (UFM1) and Its Target UFBP1 Protect Pancreatic Beta Cells from ER Stress-Induced Apoptosis PLoS One 2011 [PMID:21494687] (IP)	IP
Dickerhof N, Kleffmann T, Jack R, McCormick S. Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain FEBS Journal 2011 [PMID:21481187] (IA)	IA
Knudsen T, Kjelgaard-Hansen M, Tranholm M et al. Canine specific ELISA for coagulation factor VII The Veterinary Journal 2011 [PMID:21216638] (IA)	IA
Iankov ID, Haralambieva IH, Galanis E. Immunogenicity of attenuated measles virus engineered to express Helicobacter pylori neutrophil-activating protein Vaccine 2010 [PMID:21182995] (IA)	IA
Howes MT, Kirkham M, Riches J et al. Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells Journal of Cell Biology 2010 [PMID:20713605]
Van Dessel N, Beke L, Gornemann J et al. The Phosphatase Interactor NIPP1 Regulates the Occupancy of the Histone Methyltransferase EZH2 at Polycomb Targets Nucleic Acids Research 2010 [PMID:20671031]
Kim JY, Xin X, Moioli EK et al. Regeneration of Dental-Pulp-like Tissue by Chemotaxis-Induced Cell Homing Tissue Eng Part A 2010 [PMID:20486799] (IA)	IA
Stubenrauch K, Wessels U, Essig U et al. Detection of drug specific Circulating Immune Complexes from in vivo Cynomolgus Monkey Serum Samples. J Immunol Methods. 2014 [PMID:20083366]
Salipante SJ, Rojas ME, Korkmaz B et al. Contributions to Neutropenia from PFAAP5 (N4BP2L2), a Novel Protein Mediating Transcriptional Repressor Cooperation between Gfi1 and Neutrophil Elastase Molecular and Cellular Biology 2009 [PMID:19506020] (IB)	IB
Mills DR, Haskell MD, Callanan HM et al. Monoclonal antibody to novel cell surface epitope on Hsc70 promotes morphogenesis of bile ducts in newborn rat liver Cell Stress and Chaperones 2010 [PMID:19415527] (IB)	IB
Monfils MH, Cowansage KK, Klann E et al. Extinction-Reconsolidation Boundaries: Key to Persistent Attenuation of Fear Memories Science 2009 [PMID:19342552] (IA)	IA
Al-Dujaili EA, Mullins LJ, Bailey MA, Kenyon CJ. Development of a Highly Sensitive ELISA for Aldosterone in Mouse Urine: Validation in Physiological Pathophysiological States of Aldosterone Excess Depletion -. Steroids. 2008 [PMID:19162057]
Bao S, Wu Q, Li Z, Sathornsumetee S, Wang H, McLendon RE, HjelmelAB, Rich JN. Targeting Cancer Stem Cells Through L1CAM Suppresses Glioma Growth -. Cancer Research, 68 pp 6043-6048. 2008 [PMID:18676824]
Marr AK, Jenssen H, Moniri MR, Hancock RE, Pante N. Bovine lactoferrin lactoferricin interfere with intracellular trafficking of Herpes simplex virus-1. Biochimie, 91 (1) pp 160-164. 2009 [PMID:18573311]
Velappan N, Clements J, Kiss C, Valero-Aracama R, Pavlik P, Bradbury AR. Fluorescence Linked Immunosorbant Assays Using Microtiter Plates. Journal of Immunological Methods, 336 pp 135-141. 2008 [PMID:18514691]
Show All 64 Publications. Collapse Publications.

Reviews for Lightning-Link HRP Kit (701-0003) (3) 4.33

Average Rating: 4.3 (Based on 3 reviews) We have 3 reviews tested in 2 species: Human, Other. We have 3 reviews tested in 2 application: ELISA, WB. Submit a Review Reviews using 701-0003: Filter by Applications ELISA (1) WB (2) All Applications Filter by Species Human (2) Other (1) All Species

Images	Ratings	Applications	Species	Date	Details
Â	reviewed by:Fugui Li	ELISA	Human	09/23/2014	View Summary Application ELISA Sample Tested purified monoclonall antibody Species Human
Enlarge	reviewed by:Robert Hnasko	WB	Other	04/18/2011	View Summary Application Western Blot Sample Tested recombinant protein, Sample Amount: 100ng Species Other Comments Fast, efficient, Stable, works as described with large scale - excellent product Blocking Blocking Details Blocking Buffer: 10% NFDM in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature Primary Anitbody Dilution Ratio Dilution Ratio: 1ug/mL, Incubation Dilution Buffer: TBST + 0.05% IgG free BSA, Incubation Time: 30 min, Incubation Temp: RT Details Detection Notes Detection Method: ECL, Exposure Time: 20 sec, Positive Control: recombinant protein, Specific Bands: Yes, Non-Specific Bands: No Comments Comments Fast, efficient, Stable, works as described with large scale - excellent product
Â	reviewed by:	WB	Human	06/10/2010	View Summary Application Western Blot Sample Tested Purified scFv protein Species Human Lot 1232 Comments The product is good overall, but our background was high, and the conjugation (molar ratios,etc.) needs to be optimized. Blocking Blocking Details Blocking Buffer: TBST+5%Milk, Blocking Time: 1 hour, Blocking Temp: 73 degrees Fahrenheit Primary Anitbody Dilution Ratio Dilution Ratio: 1:500, Incubation Dilution Buffer: TBST+5%Milk, Incubation Time: 60 minutes, Incubation Temp: 73 Fahrenheit Secondary Antibody Secondary Description donkey anti-rabbit IgG-HRP Details Detection Notes Detection Method: ECL+, Exposure Time: 10 seconds, Specific Bands: 46 kDa, Non-Specific Bands: 55, 37, 30, 25 kDa Comments Comments The product is good overall, but our background was high, and the conjugation (molar ratios,etc.) needs to be optimized.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

• Western Blot
• Immunohistochemistry-Paraffin
• View all Protocols, Troubleshooting, Illustrated assays and Webinars.

Video Protocols

WB Video Protocol

FAQs for Lightning-Link HRP Kit (701-0003). (Showing 1 - 10 of 10 FAQs).

1. What type of volume can be run with a 5 by 1mg or 1 by 5mg vial of LL-HRP?
   • The optimal concentration range for the antibodies to be labeled is between 1.0-2.5 mg/mL.
2. Our customer would like to know how to evaluate the amount of HRP conjugated successfully on antibodies?
   • The amount of HRP conjugated is usually determined by the molar ratio used in the conjugation reaction. This easy approach is possible because – provided the protocols are followed – Lightning-Link reactions are very efficient, resulting in very high recovery. HRP is about 1/4 the size of an antibody (approx. 40 kDa versus approx. 160 kDa). Therefore, if 100 ug antibody is added to 100 ug HRP, there will be an average of 4 HRP molecules bound to each antibody molecule, and if 400 ug antibody is added to 100 ug HRP, there will be an average of 1 HRP molecule bound to each antibody molecule (and so on).
3. Can I label a peptide/protein/antibody other than IgG?
   • Yes. The Lightning-Link® technology works by targeting free amine groups on your target, meaning it can be used to label most biomolecules.As the protocols provided were optimized for labeling IgGs, we would recommend you adjust the amount of material you add to the Lighting-Link® vial to allow for molecular weight difference. This should be done without changing the volume added to the vial, as this could affect the conjugation efficiency. As a rough guideline, we would recommend changing the amount of material proportionally to the size difference with IgGs. An average IgG is about 160kD, therefore for a target that is half the size of an antibody (about 80kD), add half as much to the vial. Please note this is only a guideline and the best amount for your assay should be determined experimentally; our 3x10ug kits enable you to do this using small amounts of material and therefore at a low cost.
4. Do I need a wash or desalt step?
   • One of the advantages of the Lightning-Link® technology compared to traditional labeling methods is that conjugate purification is not required. The Lightning-Link® reactions are highly efficient, and the kits have been optimized so that, provided the protocols are followed, there should be only a very low amount of free label left at the end of the conjugation. Any remaining free label would have its reactive 'Lightning-Link®' groups blocked by the Quencher provided in the kit, and would then be washed away during the relevant wash step of your application.
5. Do my antibody and buffer fit the requirements?
   • Your antibody should be purified (affinity purification is preferred), as other molecules with free amine groups will interfere with the reaction, resulting in a poor quality conjugate. A suitable method of purification should be used (such as affinity or Protein A/G). Please note that 0.2/0.22 um filtration is a method of sterilization, not purification.Your biomolecule should also be in a suitable, 10-50mM amine free buffer (e.g. MES, MOPS, HEPES, PBS), pH range 6.5 to 8.5 and not in any of the following: ascites fluid, serum or tissue culture supernatant. It should not contain any additives such as Azide, BSA, Tris or Glycine at a concentration of >0.1%.The amount of antibody (IgG) you should add to the Lightning-Link® vial usually corresponds to the kit size your purchased (for example: a 3x100ug kit enable you to label 3 lots of 100ug antibody) and the volume added should also match (eg: 100ul), meaning the ideal concentration is of 1mg/ml. For other biomolecule, the amount added should be adjusted by changing your concentration (see above).NOTE: The amount and volume of antibody recommended above are for all Lightning-Link• kits offered by Innova Biosciences, with the exception of RPE and PE tandem dyes. For more details on the recommendations specific to these dyes, please consult the protocols available on each product page.
6. How many labels will bind to my biomolecule?
   • It is difficult to give a precise answer to this as the result will vary from antibody to antibody (or other protein). Traditional labelling techniques often have F/P ratios in the range of 4-7:1, and our comparative data for staining with antibodies labelled with Lightning Link shows similar staining intensity. The ratio of dye to antibody is only ever an average for the population of labelled molecules, as individual molecules may have different amount of dye incorporated into them.
7. How stable will my new conjugate be?
   • The Lightning-Link® chemistry joins the label to the antibody via a uni directional stable, covalent bond. The stability of your conjugate will therefore be dependent on your antibody and label of choice. In our in-house studies, conjugates made with our Lightning-Link® kits were fully active after more than 18 months' storage, undiluted, at 4 degrees C. Conjugates stored in 50% glycerol at -20 degrees C were found to be even more stable (several years). Please see below for advice on storage conditions. Please note that tandem dye stability is lower than the other dyes'. Tandem conjugates are fully stable for about 3 months at 4 degrees C.
8. What are the best storage conditions for my new conjugate?
   • TA new conjugate can be stored for 12-18 months at 4 degrees C as long as the antibody will tolerate storage at 4 degrees C. As the bond between the antibody and dye is covalent and very stable, it should not degrade, therefore at 4 degrees C no additional preservatives are needed. The antibody is usually the least stable component of the conjugate so please check the antibody datasheet for storage recommendations. However, all conjugates can be stored in 50% glycerol at -20 degrees C which allows them to remain stable for 2 years. Please bear in mind that APC and RPE conjugates should never be stored at -20 degrees C on their own without glycerol. The dilution factor also has an effect. Storing the conjugate undiluted is recommended if possible; however, for HRP conjugates, if you wish to store at working concentrations (e.g. 1/10,000), this can be done using our LifeXtend HRP Conjugate Stabilizer/Diluent. Please note that the preservative sodium azide will inhibit HRP. We suggest that the optimal storage conditions for any conjugate are determined experimentally, using small aliquots of the conjugate.
9. What can I do if my antibody formulation does not fit the requirements?
   • If your antibody buffer is not compatible with our kits, we have developed an AbSelectâ„¢ purification kit range that allows you to quickly and simply purify your antibody and is fully compatible with the Lightning-Link® kits.The appropriate kit to use depends on your particular sample (species, buffer, contaminants, volume,...). We have designed a handy flow chart on the AbSelectâ„¢ webpage to help you select a kit, but feel free to contact us if you require further guidance. Please consult the kit protocols to see the antibody amount/volume suitable for each kit.If your antibody is already purified but its concentration is too low, you can concentrate it by using our Antibody Concentration and Clean Up Kit. This kit can also be used to remove low molecular weight contaminants such as azide, tris or glycine by carrying out a buffer exchange into the buffer supplied in the kit, which is fully compatible with Lightning-Link®.If your antibody contains BSA, you can now use our BSA removal kit to purify your antibody in a few simple steps. Please note this kit will also enable you to concentrate your antibody.NB: All the AbSelect kits will ONLY work with antibodies. They will not purify other molecules. The only exception is the concentration and clean up kit (861-0010), which will work with other molecules greater than 10kD.
10. What is the difference between Lightning-Link® and Lightning-Link® Rapid?
    • The benefit of the Rapid kits is that the conjugates can be generated faster because the conjugation and quenching incubation times are shorter. The antibody/protein/peptide considerations are exactly the same, as is the high quality of the final conjugate. DyLight® dyes are only available in the Rapid format.

Additional Lightning-Link HRP Products

Lightning-Link HRP 701-0003

• Lightning-Link HRP Kits

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