Novus Maleimide Activated BSA, 5 mg Item ID: NOV4080005

Maleimide Activated BSA Summary

Description

Maleimide activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (-SH) group. The reactive maleimide groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance. Features Benefits Compatible protein thiolation kit available No specialist knowledge required Covalent bond Highly stable conjugates Maleimide activated labels can be used with a range of biomolecules, including oligonucleotides Flexible Scalable Easy transfer from R&D to manufacturing Stringently QC tested Consistent high quality, excellent batch-to-batch reproducibility Traditional, well-known conjugation chemistry Trusted method Maleimide activated labels Maleimide - RPE Maleimide - HRP Maleimide - Alkaline Phosphatase Maleimide - APC Maleimide-Streptavidin Maleimide-Ovalbumin Maleimide-BSA Maleimide - KLH If the biomolecule does not have a free thiol group, it will require activation to generate a free thiol group prior to conjugation with the maleimide label:1. Protein Thiolation Kit Designed to facilitate the conjugation of proteins to our maleimide-activated labels. Simply add your protein solution to the lyophilized thiolation reagents to activate your protein. These kits are designed to activate 2mg or 5mg of your protein.Each thiolation kit is also supplied with a thiolation detection kit provided as a positive test for protein thiolation, giving you the option to confirm the chemistry is working.2. Thiol Quantification Kit The thiol quantification assay kit allows you to quantify the amount of thiol/sulfhydryl (-SH) groups in samples of thiolated proteins or antibodies. Quantifying the number of free thiols in a sample can be useful in a number of applications and allows for a more controlled conjugation reaction.The assay is in a convenient 96-well plate format and contains all the necessary reagents required.

Specificity

Maleimide-activated BSA is designed to facilitate the conjugation of the BSA (bovine serum albumin) to proteins, peptides and ligands that contain sulfhydryl (-SH) groups. The reactive maleimide group that has been added to BSA allows conjugation reactions to be performed very efficiently at physiological pH.

Packaging, Storage & Formulations

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	Supplied as freeze dried formulations
Preservative	No Preservative

Applications/Dilutions

Dilutions	Immunohistochemistry 1:10-1:500
Publications	Read Publication using 408-0005.

Notes

Molecules that do not contain -SH groups can be chemically modified to introduce the required thiol functionality. In the case of antibodies, disulfide (S-S) bridges may also be cleaved to release free thiols. This prodcut is manufacture by Innova Biosciences.

Background

Maledimide and periodate activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (-SH) or amine (-NH2) group respectively. The reactive maleimide or periodate groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance. Innova Biosciences activated labels are supplied as stable freeze dried formulations, with all material being rigorously QC tested using thiolated biomolecules to ensure high conjugation efficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.