Novus Lightning-Link Rapid Type A Biotin Antibody Labeling Kit, 3 Reactions (up to 20 ug) Item ID: NOV3700030

Lightning-Link Rapid Type A Biotin Antibody Labeling Kit Summary

Description

Lightning-Link Rapid Antibody Labeling kits enable direct labeling of antibodies, proteins, peptides or other biomolecules and allow conjugations to be set up in seconds, and used within minutes (less than 20 minutes). Our Lightning-Link Rapid technology can be used to label small quantities of antibody with 100% recovery. The researcher simply pipettes the antibody or other biomolecule into a vial of lyophilized mixture containing the label of interest and incubates the reaction for only 15 minutes (for more details please watch the video below). As there are no desalting or dialysis steps that commonly interrupt traditional protein labeling procedures. Features Benefits Quick and easy to use Save time, no special knowledge required No separation steps 100% recovery - no antibody/protein loss Can be used in a wide range of applications Flexible Freeze dried Ships at ambient temperature, long shelf-life Fully scalable (10 ug to 1 g or more) Easy transfer from R&D to manufacturing Stringently QC tested Consistent high quality, excellent batch-to-batch reproducibility Large number of labels available Experimental flexibility Reliable: nearly 300 references Successfully used in many fields of research The attachment of biotin to biomolecules is an important laboratory technique. Biotin binds to the tetrameric avidin proteins, including streptavidin and neutravidin, with exceptionally high affinity, and this interaction is exploited in various applications such as western blotting, immunohisthochemistry and ELISA. Our biotinylation kits are optimised to create high quality conjugates and minimise assay background. Lightning-Link Biotin Has been optimized for two separate applications: Type A - Intended for assays in which a streptavidin-labeled detection reagent will be used. Type B - Optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).

Kit Type

Antibody Labeling Kit

Packaging, Storage & Formulations

Storage

Store at -20C. Avoid freeze-thaw cycles.

Applications/Dilutions

Application Notes

The technology is fully scalable (ug to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.

Kit Components

Components

1 or 3 glass vial(s) of Lightning-Link Rapid mix 1 vial of LL-Rapid Modifier reagent 1 vial of LL-Rapid Quencher reagent

Notes

Learn more about Lightning-Link™ Conjugation Kits by reading FAQsFor more information please check out these useful links!Fluorescent Labels Poster Antibody Labeling GuideAntibody Purification GuideLightning Link® is a registered trademark of Innova Biosciences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.