D10.G4.1; T Lymphocyte; Mouse (Mus musculus) Item ID: ATCTIB224
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Mus musculus, mouse |
| Tissue | lymph node |
| Cell Type | helper T lymphocyte (Th-2) |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | adult |
| Gender | male |
| Strain | AKR/J |
| Applications | D10.G4.1 has been used extensively to study activation of T cells. These cells can also be used as a reliable and sensitive assay for IL-1 (interleukin-1) response, since D10 cells will proliferate only minimally to con A in the absence of IL-1 or feeder cells. |
| Storage Conditions | liquid nitrogen vapor phase |
| Clinical Data | male |
|---|---|
| Antigen Expression | H-2k |
| Receptor Expression |
interleukin 1 (IL-1), expressed; interleukin 1 (IL-1) |
| Genes Expressed | interleukin 4 (interleukin-4, IL-4); interleukin 5 (interleukin-5, IL-5) |
| Cellular Products | interleukin 4 (interleukin-4, IL-4); interleukin 5 (interleukin-5, IL-5) |
| Comments | The cells are specific for egg white conalbumin (Sigma No. C0755) and alloreactive to I-A b, v. Supernatant media from Con A stimulated D10.G4.1 cells contains IL-4 and IL-5. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
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| Subculturing | Subculture actively growing suspension cultures before they have reached 1 X 106 cells/mL or the IL-1 and IL-2 will be rapidly depleted and the cells will lose viability. Use inoculation densities of 2 X 105 viable cells/mL. Medium Renewal: Twice per week |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with an additional 10% FBS and 5% (v/v) DMSO. Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor | C Janeway, J Kaye, P Conrad |
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| References |
Kupper T, et al. Growth of an interleukin-2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). J. Immunol. 138: 4288-4292, 1987. PubMed: 2953804 Kaye J, Janeway CA J. Induction of receptors for interleukin 2 requires T cell Ag:Ia receptor crosslinking and interleukin 1. Lymphokine Res. 3: 175-182, 1984. PubMed: 6436584 Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J. Exp. Med. 150: 1510-1519, 1979. PubMed: 92524 Kaye J, et al. Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells. J. Exp. Med. 158: 836-856, 1983. PubMed: 6193236 Kaye J, Janeway CA Jr.. The Fab fragment of a directly activating monoclonal antibody that precipitates a disulfide-linked heterodimer from a helper T cell clone blocks activation by either allogeneic Ia or antigen and self-Ia. J. Exp. Med. 159: 1397-1412, 1984. PubMed: 6232337 Kaye J, et al. Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: interleukin 1 is required for the expression of receptors for interleukin 2. J. Immunol. 133: 1339-1345, 1984. PubMed: 6235287 Kaye J, Janeway CA Jr.. The alpha- and beta-subunits of a murine T cell antigen/Ia receptor have a molecular weight of 31,000 in the absence of N-linked glycosylation. J. Immunol. 133: 2291-2300, 1984. PubMed: 6237148 Swain SL, et al. Role of antigen in the B cell response. Specific antigen and the lymphokine IL-5 synergize to drive B cell lymphoma proliferation and differentiation to Ig secretion. J. Immunol. 140: 4224-4230, 1988. PubMed: 2453572 |
| Permits |
These permits may be required for shipping this product to Australia:
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|---|---|
| Basic Documentation | Product Sheet Certificate of Analysis SDS |
| References |
Kupper T, et al. Growth of an interleukin-2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). J. Immunol. 138: 4288-4292, 1987. PubMed: 2953804 Kaye J, Janeway CA J. Induction of receptors for interleukin 2 requires T cell Ag:Ia receptor crosslinking and interleukin 1. Lymphokine Res. 3: 175-182, 1984. PubMed: 6436584 Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J. Exp. Med. 150: 1510-1519, 1979. PubMed: 92524 Kaye J, et al. Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells. J. Exp. Med. 158: 836-856, 1983. PubMed: 6193236 Kaye J, Janeway CA Jr.. The Fab fragment of a directly activating monoclonal antibody that precipitates a disulfide-linked heterodimer from a helper T cell clone blocks activation by either allogeneic Ia or antigen and self-Ia. J. Exp. Med. 159: 1397-1412, 1984. PubMed: 6232337 Kaye J, et al. Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: interleukin 1 is required for the expression of receptors for interleukin 2. J. Immunol. 133: 1339-1345, 1984. PubMed: 6235287 Kaye J, Janeway CA Jr.. The alpha- and beta-subunits of a murine T cell antigen/Ia receptor have a molecular weight of 31,000 in the absence of N-linked glycosylation. J. Immunol. 133: 2291-2300, 1984. PubMed: 6237148 Swain SL, et al. Role of antigen in the B cell response. Specific antigen and the lymphokine IL-5 synergize to drive B cell lymphoma proliferation and differentiation to Ig secretion. J. Immunol. 140: 4224-4230, 1988. PubMed: 2453572 |