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Primary Peripheral Blood Mononuclear Cells, Normal, Human Item ID: ATCPCS800011

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type mononuclear
Morphology spherical; variable after culturing
Growth Properties suspension; variable after culturing
Biosafety Level 1

 [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens. 

ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.] 

Human Material Precaution  All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease normal
Age lot specific
Gender lot specific
Ethnicity lot specific
Applications Applications for use include the study of immunology, infection, cancer, hematology, and t-cell suppression assay.
Product Format frozen 1 mL
Storage Conditions -130°C
Images
Comments Peripheral blood mononuclear cells (PBMC) have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining peripheral blood mononuclear cells in culture in the absence of application-specific growth factors. 

Peripheral blood mononuclear cells are a heterogeneous population of blood cells with a single round nucleus and include macrophages, dendritic cells, monocytes and lymphocytes.
Complete Growth Medium Peripheral blood mononuclear cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining peripheral blood mononuclear cells in culture in the absence of application-specific growth factors.

Note: Application specific medium, cytokines and other factors
Subculturing N/A
Volume 1 mL
Cells per Vial One vial contains a minimum of 2.5 x 107 viable cells.
Viral Testing Hepatitis B: Negative Hepatitis C: Negative HIV(I/II): Negative HTLV(I/II): Negative WNV: Negative Trypanasome: Negative
Viability ≥ 70% when thawed from cryopreservation
Population Doubling Capacity N/A
C of A Certificate of Analysis
Permits

These permits may be required for shipping this product to Australia:

  • DAFF Import Permit formerly known as AQIS Import Permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment.
Basic Documentation Product Sheet Certificate of Analysis SDS
Other Documentation PCS-800-011 T-Suppression Application
FAQs
  1. Thawing ATCC® PCS-800-011 ™, Human Peripheral Blood Mononuclear (PBMC) and ATCC® PCS-800-013 ™, Human Bone Marrow Mononuclear cells

    We recommend the following thawing procedure:

    Date Updated: 8/18/2014
  2. Culture medium for PCS-800-011 and PCS-800-013ATCC does not recommend maintaining mononuclear cells in culture in the absence of application-specific growth factors. We have found that mononuclear cells can however, be maintained in un-suppl...Date Updated: 8/18/2014
  3. Subpopulations in ATCC® PCS-800-011 ™, Human Peripheral Blood Mononuclear (PBMC) and ATCC® PCS-800-013 ™, Human Bone Marrow Mononuclear cells

    Date Updated: 8/18/2014

  4. Cryopreservation medium of PCS-800-011 and PCS-800-013 cellsATCC mononuclear cells are cryopreserved in CryoStor® CS10 Freeze Medium from BioLife Solutions.Date Updated: 8/18/2014
  5. How are PCS-800-011 and PCS-800-013 cells obtainedATCC mononuclear cells are collected from either peripheral blood or bone marrow and isolated using density gradient centrifugation methods.Date Updated: 8/18/2014
  6. PCS-800-011 and PCS-800-013 donor sourceNo, a single vial of ATCC mononuclear cells contains only material sourced from a single donor.Date Updated: 8/18/2014

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