pGRN145 plasmid; in E. coli GC10 Item ID: ATCMBA141
| Permits and Restrictions |
View Permits View Restrictions |
|---|---|
| Designations | pGRN145 plasmid in E. coli GC10 |
| Depositors | Geron Corporation |
| Applications | Expression vector [telomerase reverse transcriptase] (EST2; TCS1; TP2; TRT; hEST2; telomerase catalytic subunit) |
| Vector |
Total size (kb): 14.002
Bacterial selection marker: cmlR
Features: hTERT: 4225-7623 HygB: 9216-10238 (complementary) CatR: 10822-11480 AmpS: 12941-13799 (complementary) PAC: 368-964 (complementary) F1 ori: 11501-11685 (complementary) MPSV: 3643-4119 (promoter) Ori: 12165-12747
Restriction digests of this clone gave the following sizes (in kb): HindIII/KpnI - 10.5, 3.5; EcoRI - 10.6, 3.4; XhoI - 12.0, 2.0. |
| Insert | Gene: Telomerase reverse transcriptase (TERT) Source: Human Insert size (kb): 3.398 GenBank accession: NM_003219 |
| Media | ATCC® Medium 2585: LB Medium (ATCC medium 1065) with 37 mcg/ml chloramphenicol |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information | Frozen glycerol stock of E. coli GC10 containing the plasmid |
| Bascic Documentation | Product Sheet Certificate of Analysis SDS |
|---|---|
| Restrictions |
This material is subject to claims under U.S. Patent Nos. 6,261,836 and 6,337,200, other pending patent applications, and foreign counterparts thereof. It is required that the Addendum for Noncommercial and Academic Organizations be signed and returned to ATCC before shipment. Commercial and For Profit Organizations need to obtain a license from Geron Corporation by contacting info@geron.com before shipment. |
| Attachments | MSDSFigure 1Figure 2Figure 3 |