In Vitro Technologies

Permits and Restrictions	View Permits View Restrictions
Organism	Homo sapiens, human
Tissue	lung; derived from metastatic site: bone marrow
Cell Type	Epithelial
Product Format	frozen
Morphology	epithelial
Culture Properties	suspension, multicell aggregates
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	carcinoma; small cell lung cancer
Age	59 years
Gender	male
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase

Karyotype	This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. At least 15 markers were common to all cells including single copies of der(1)t(1;13)(p32;q12) and del(3)(p21/22), and paired der(7)t(7;?)(p22;?), t(13q16q) and t(16p21q). Ten others were seen in single copies or paired. No HSR or DM were found. Structurally normal N5, N13 and N14 were absent. Four or more copies of N18 and N20 were present in each cell, and N5, N7 and N11 were mostly single copies per cell. The X chromosomes were paired, and no Y chromosome was detected in QM stained preparations.
Clinical Data	59 years Caucasian male
Receptor Expression	insulin-like growth factor II (IGF II)
Oncogene	myc +; myb +; raf +; ras +; fms +; fes -
Genes Expressed	The cells do not express vasopressin, oxytocin or gastrin releasing peptide. The cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein. There is expression of c-myb, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs. Oncogenes: myc +; myb +; raf +; ras +; fms +; fes -
Tumorigenic	Yes
Effects	Yes, in nude mice
Comments	The cells produce relatively high amounts of c-myc mRNA, but c-myc DNA sequences are not amplified. There is expression of c-myb, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs. The line expresses elevated levels of four biochemical markers (neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	The line should be subcultured by dilution with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C

STR Profile	Amelogenin: X CSF1PO: 11 D13S317: 11,12 D16S539: 11 D5S818: 12 D7S820: 9,10 THO1: 6,9.3 TPOX: 8,11 vWA: 14,16
Isoenzymes	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 2 PGM1, 1-2 PGM3, 1-2

Name of Depositor	AF Gazdar, JD Minna
Year of Origin	1979
References	Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876 Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917 Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141 Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257 Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028 Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370 Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893 Lung Cancer 4: 155-161, 1988. The NCI-H146 cell line was derived by A.F. Gazdar and associates in 1979 from a bone marrow metastasis of a patient with small cell cancer of the lung. The bone marrow specimen was taken prior to therapy. The cells form transplantable tumors which histologically resemble tumor cells from the original biopsy specimen.

Name of Depositor

AF Gazdar, JD Minna

Year of Origin

1979

References

Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917

Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028

Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370

Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893

Lung Cancer 4: 155-161, 1988.

The NCI-H146 cell line was derived by A.F. Gazdar and associates in 1979 from a bone marrow metastasis of a patient with small cell cancer of the lung. The bone marrow specimen was taken prior to therapy.

The cells form transplantable tumors which histologically resemble tumor cells from the original biopsy specimen.

Permits	These permits may be required for shipping this product to Australia: DAFF Import Permit formerly known as AQIS Import Permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
Restrictions	The line is available with the following restrictions: This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. Any proposed commercial use of the these cells, or their products, must first be negotiated with the National Cancer Institute (NCI). For further information, please contact NCI’s Technology Transfer Center at NCI_TTC_Contact@mail.nih.gov or by phone at (240)-276-5514.
References	Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876 Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917 Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141 Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257 Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028 Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370 Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893 Lung Cancer 4: 155-161, 1988. The NCI-H146 cell line was derived by A.F. Gazdar and associates in 1979 from a bone marrow metastasis of a patient with small cell cancer of the lung. The bone marrow specimen was taken prior to therapy. The cells form transplantable tumors which histologically resemble tumor cells from the original biopsy specimen.

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P: 1300 552 003

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NCI-H146; Small Cell Lung Cancer; Human (Homo sapiens) Item ID: ATCHTB173

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