SW 1088; Astrocytoma; Human (Homo sapiens) Item ID: ATCHTB12
| Permits and Restrictions |
View Permits |
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| Organism | Homo sapiens, human |
| Tissue | brain |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | astrocytoma |
| Age | 72 years |
| Gender | male |
| Ethnicity | Caucasian |
| Karyotype | hypertriploid; modal number = 72 to 74. The rate of higher ploidies was 4.2%. Most chromosomes were morphologically normal. Three marker chromosomes were common to all cells: del(1) (q11), der (9)t(7;?;9) (q11?;?;p24), and der (10)t(4;10) (q21;q15)., The der (9) was paired in nearly 50% of the cells. Usually one, but occasionally three double minutes (DM) were seen in a few cells. Five copies of normal N5, N7 and N20 were seen in most cells., The X and Y were paired. The presence of Y chromosomes was confirmed in the QM stained preparation. |
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| Derivation | The SW 1088 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1975 from an astrocytoma taken from a 72 year old male Caucasian. The cell line was received at the ATCC in January, 1982 at passage 23. |
| Clinical Data | 72 years Caucasian male |
| Antigen Expression | Blood type A; Rh+ |
| Tumorigenic | Yes |
| Effects | Yes, in nude mice |
| Comments | The cells produce a grade III tumor. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
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| Subculturing |
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommendedMedium Renewal: 2 to 3 times per week |
| Cryopreservation | Culture medium, 95%; DMSO, 5% |
| Culture Conditions | Atmosphere: air, 100% |
| STR Profile | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 12 D16S539: 12,13 D5S818: 11 D7S820: 9 THO1: 7,9.3 TPOX: 8 vWA: 16,17 |
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| Isoenzymes | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1-2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor | A Leibovitz |
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| Passage History | The cell line was received at the ATCC in January, 1982 at passage 23. |
| References |
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474 |
| Cross References |
Nucleotide (GenBank) : AF188519 AF188519 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone ISG 1, mRNA sequence. Nucleotide (GenBank) : AF188520 AF188520 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone ASM 3, mRNA sequence. Nucleotide (GenBank) : AF188521 AF188521 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone ISG 2, mRNA sequence. Nucleotide (GenBank) : AF188522 AF188522 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone ISG 3, mRNA sequence. Nucleotide (GenBank) : AF188523 AF188523 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone ISG 4, mRNA sequence. Nucleotide (GenBank) : AF188524 AF188524 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone ISG 5, mRNA sequence. Nucleotide (GenBank) : AF188525 AF188525 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone IRIG 1, mRNA sequence. Nucleotide (GenBank) : AF188526 AF188526 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone IRIG 2, mRNA sequence. Nucleotide (GenBank) : AF188527 AF188527 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone IRIG 3, mRNA sequence. Nucleotide (GenBank) : AF188528 AF188528 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone IRIG 4, mRNA sequence. Nucleotide (GenBank) : AF188529 AF188529 Homo sapiens ATCC HTB-12; SW1088 Homo sapiens cDNA clone IRIG 5, mRNA sequence. |
| Permits |
These permits may be required for shipping this product to Australia:
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| Basic Documentation | Product Sheet Certificate of Analysis SDS |
| Other Documentation | Cancer Cell Line Mutation Data Cancer Cell Line Mutation Data |
| References |
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474 |