WBC264-9C; Hybridoma; Human (Homo sapiens)/Mouse (Mus musculus) Item ID: ATCHB8902
| Organism | human (leukocyte); mouse (macrophage) |
|---|---|
| Tissue | peripheral blood |
| Cell Type | Macrophage,Macrophage-like |
| Product Format | frozen |
| Morphology | macrophage |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain: BALB/c (macrophage) |
| Applications | This is a macrophage-like cell line derived by fusion of normal human peripheral blood leukocytes with the mouse macrophage cell line, RAW 264. The cells exhibit chemotaxis to N-formyl-methionine-leucine-phenylalanine (fMet-Leu-Phe, FMLP). |
| Storage Conditions | liquid nitrogen vapor temperature |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype | at passage 6, the mean number of chromosome was 64 with a range of 36 to 81 |
|---|---|
| Derivation | This is a macrophage-like cell line derived by fusion of normal human peripheral blood leukocytes with the mouse macrophage cell line, RAW 264. |
| Comments | This is a macrophage-like cell line derived by fusion of normal human peripheral blood leukocytes with the mouse macrophage cell line, RAW 264. The cells exhibit chemotaxis to N-formyl-methionine-leucine-phenylalanine (fMet-Leu-Phe, FMLP). WBC264-9 cells express human HPRT. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing | Protocol: Subcultures are prepared by scraping. DO NOT USE TRYPSIN! Remove old medium, add fresh, dislodge cells and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions | Temperature: 37.0°C |
| Name of Depositor | The United States of America |
|---|---|
| U.S. Patent Number | 4,970,162 |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| References |
Aksamit RR. Human-mouse hybrid cell line expressing monocyte-macrophage properties. US Patent 4,970,162 dated Nov 13 1990 Aksamit RR. A human-mouse hybrid cell line that stably expresses chemotaxis to N- formylmethionyl-leucyl-phenylalanine. Biochem. Biophys. Res. Commun. 138: 1001-1008, 1986. PubMed: 3741419 |
| Basic Documentation | Product Sheet Certificate of Analysis SDS |
|---|---|
| References |
Aksamit RR. Human-mouse hybrid cell line expressing monocyte-macrophage properties. US Patent 4,970,162 dated Nov 13 1990 Aksamit RR. A human-mouse hybrid cell line that stably expresses chemotaxis to N- formylmethionyl-leucyl-phenylalanine. Biochem. Biophys. Res. Commun. 138: 1001-1008, 1986. PubMed: 3741419 |