In Vitro Technologies :: Products

Permits and Restrictions	View Permits View Restrictions
Organism	Homo sapiens, human
Product Format	frozen
Culture Properties	mixed, adherent and suspension (floating clusters in suspension with some adherent cells)
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	stage E,carcinoma; small cell lung cancer
Age	54 years
Gender	male
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase

Clinical Data	male Caucasian 54 years

Complete Growth Medium	HITES medium supplemented with 5% fetal bovine serum The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) extra 2mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.)
Subculturing	Protocol: Cultures can be maintained by addition of fresh medium. Attached cells can be scaped. Medium Renewal: two to three times weekly
Cryopreservation	Freeze medium: Culture medium, 95%; additional fetal bovine serum, 5%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C

Name of Depositor

AF Gazdar, JD Minna

Year of Origin

August, 1989

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

70 pack years

floating clusters in suspension with some adherent cells

floating clusters in suspension with some adherent cells

E: care@invitro.com.au
P: 1300 552 003

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