NCI-H1385; Lung Carcinoma; Human (Homo sapiens) Item ID: ATCCRL5867
| Permits and Restrictions |
View Permits View Restrictions |
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| Organism | Homo sapiens, human |
| Tissue | lung; derived from metastatic site: lymph node |
| Product Format | frozen |
| Culture Properties | suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | stage 3A, carcinoma; non-small cell lung cancer |
| Age | 49 years |
| Gender | female |
| Ethnicity | Black |
| Storage Conditions | liquid nitrogen vapor phase |
| Derivation | The line was established in April 1986 from lymph node, a metastatic site. |
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| Clinical Data | 49 years old female Black The patient was a smoker, 33 packs year. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
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| Subculturing | This line grows as aggregates of cells in suspension. Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium.Medium Renewal: 2 times per week |
| Cryopreservation | Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 9,11 D5S818: 13 D7S820: 8,10 THO1: 7 TPOX: 8,9 vWA: 17,19 |
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| Name of Depositor | AF Gazdar, JD Minna |
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| Year of Origin | 1986 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |