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Permits and Restrictions	View Permits View Restrictions
Organism	Homo sapiens, human
Tissue	lung
Cell Type	lymphoblast
Product Format	frozen
Culture Properties	suspension, The cells grow as floating aggregates of round cell clusters.
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	stage L,carcinoma; small cell lung cancer
Age	42 years
Gender	male
Ethnicity	Caucasian
Applications	The line was established in September 1985.

Karyotype	del(p13-p21)
Derivation	The line was established in September 1985.
Clinical Data	The patient was a smoker. A lymphoblastoid line from the same patient is available as ATCC CRL-5949. male Caucasian 42 years
Comments	The line was established in September 1985. Fast growing. The patient was a smoker. 75 pack years. A lymphoblastoid line from the same patient is available as ATCC CRL-5949.

Complete Growth Medium	ACL-4 medium (serum-free)The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: 0.02 mg/ml insulin 0.01 mg/ml transferrin 25 nM sodium selenite (final conc.) 50 nM Hydrocortisone (final conc.) 1 ng/ml Epidermal Growth Factor (do not filter) 0.01 mM ethanolamine (final conc.) 0.01 mM phosphorylethanolamine (final conc.) 100 pM triiodothyronine (final conc.) 0.5% (w/v) bovine serum albumin (final conc.) 10 mM HEPES 0.5 mM sodium pyruvate (final conc.) extra 2mM L-glutamine (for final conc. of 4.5mM)
Subculturing	Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively the cells may be collected by centrifugation with subsequent resuspension in fresh medium. The cells are slow to grow and the clusters will get larger over time. Some clusters can lightly adhere to the flask. These cells cannot be counted accurately, if the cell clusters are dispersed into single cells, they will lose viability. Add fresh medium every 2 to 3 days.
Cryopreservation	RPMI 1640 medium 85%; fetal bovine serum, 10%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C

Name of Depositor	AF Gazdar, JD Minna
Year of Origin	1985
References	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Permits	These permits may be required for shipping this product to Australia: DAFF Import Permit formerly known as AQIS Import Permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
Restrictions	The line is available with the following restrictions: This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. Any proposed commercial use of the these cells, or their products, must first be negotiated with the National Cancer Institute (NCI). For further information, please contact NCI’s Technology Transfer Center at NCI_TTC_Contact@mail.nih.gov or by phone at (240)-276-5514.
References	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

E: care@invitro.com.au
P: 1300 552 003

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