NCI-H1092; Lung Carcinoma; Human (Homo sapiens) Item ID: ATCCRL5855
| Permits and Restrictions |
View Permits View Restrictions |
|---|---|
| Organism | Homo sapiens, human |
| Tissue | lung; derived from metastatic site: bone marrow |
| Product Format | frozen |
| Culture Properties | suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | stage E, carcinoma; classic small cell lung cancer |
| Age | 67 years |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | del(p12-p23) |
|---|---|
| Derivation | The line was established in June 1985. |
| Clinical Data | male Caucasian 67 years oldThe patient was a smoker, 120 packs a year |
| Comments | Fast growing. |
| Complete Growth Medium |
HITES medium supplemented with 5% fetal bovine serum
|
|---|---|
| Subculturing | Cultures can be maintained by the addition of fresh medium or alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters. Medium Renewal: Add fresh medium as cell density increases. |
| Cryopreservation | Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Name of Depositor | AF Gazdar, JD Minna |
|---|---|
| Year of Origin | 1985 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |