NCI-H211; Small Cell Lung Cancer; Human (Homo sapiens) Item ID: ATCCRL5824
| Permits and Restrictions |
View Permits View Restrictions |
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| Organism | Homo sapiens, human |
| Tissue | lung |
| Product Format | frozen |
| Culture Properties | aggregate in suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | stage E, carcinoma; small cell lung cancer |
| Age | 50 years |
| Gender | female |
| Ethnicity | Caucasian, White |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | iso(3p), t(3;4)(pter-q12), t(3;11)(qter-p25) |
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| Derivation | The line was established in January 1980. |
| Clinical Data | The patient received prior chemotherapy and radiation therapy. The patient was a smoker. 35 pack years. |
| Comments | Fast growing. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
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| Subculturing |
Cultures can be maintained by addition of fresh medium. Cells grow as floating aggregates of round cell clusters which should not be broken up (the cells lose viability when aggregates are broken up).Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) |
| Cryopreservation | Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 12 D13S317: 9 D16S539: 13 D5S818: 11 D7S820: 10,12 THO1: 6 TPOX: 11 vWA: 14,18 |
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| Name of Depositor | AF Gazdar, JD Minna |
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| Year of Origin | 1980 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
| Permits |
These permits may be required for shipping this product to Australia:
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| Basic Documentation | Product Sheet Certificate of Analysis SDS |
| Restrictions |
The line is available with the following restrictions:
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| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |