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UPCI:SCC152; Squamous Cell Carcinoma; Human (Homo sapiens) Item ID: ATCCRL3240

Organism Homo sapiens, human
Tissue Derived from: hypopharynx
Cell Type squamous cell
Product Format frozen 1 mL
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 2  [Cells contain human papillomavirus (HPV) DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease squamous cell carcinoma; recurrent, grade 2
Age 47
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Images
Derivation The surgically excised tumor was minced and distributed into flasks. When growth of fibroblasts obstructed the growth of the squamous cells, the fibroblasts were removed by trypsinization. The remaining squamous cells were grown and passaged.
Clinical Data 47 years Caucasian male The donor smoked tobacco, drank alcoholic beverages, and died of his disease.
Oncogene amplification of chromosomal band 11q13, not expressed mutations of TP53 codon, not expressed
Comments

UPCI:SCC152 is from a squamous cell carcinoma of the hypopharynx. It was a recurrent tumor. The patient’s original tumor site was the base of the tongue. The UPCI:SCC152 cells are positive for Human Papilloma Virus (HPV). These cells have no TP53 mutations as assayed by sequencing the 5-8 exons of TP53. UPCI:SCC152 showed no amplification of chromosomal band 11q13 using FISH.

   The base of the tongue squamous cell carcinoma is ATCC CRL-3239, UPCI:090. These 2 HNSCC (head and neck squamous cell carcinoma) cell lines are a resource for studying the initiation, cancerization, prognosis, intervention and treatment of oral cancers.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium:
  • additional 2mM L-Glutamine
    • fetal bovine serum (FBS) to a final concentration of 10%
Seeding Density 3 x 104 viable cells/cm2
Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC® No. 30-2200) or 0.25% Trypsin – 0.53mM EDTA (ATCC® No. 30-2101) solution to remove all traces of serum which contains trypsin inhibitor.
  2. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  4. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio: 1:2 to 1:4 is recommended. Medium renewal: 2 to 3 times a week
Maintenance

The flask was seeded with cells (see specific batch information) grown and completely filled with medium at ATCC to prevent loss of cells during shipping.

  1. Upon receipt visually examine the culture for macroscopic evidence of any microbial contamination. Using an inverted microscope (preferably equipped with phase-contrast optics), carefully check for any evidence of microbial contamination.  Also check to determine if the majority of cells are still attached to the bottom of the flask; during shipping the cultures are sometimes handled roughly and many of the cells often detach and become suspended in the culture medium (but are still viable).
  2. If the cells are still attached, aseptically remove all but 5 to 10 mL of the shipping medium.  The shipping medium can be saved for reuse.  Incubate the cells at 37°C in a 5% CO2   in air atmosphere until they are ready to be subcultured.
  3. If the cells are not attached, aseptically remove the entire contents of the flask and centrifuge at 125 x g for 5 to 10 minutes.  Remove shipping medium and save.  Resuspend the pelleted cells in 10 mL of this medium and add to 25 cm2 flask.  Incubate at 37°C in a 5% CO2  in air atmosphere until cells are ready to be subcultured.
Volume 1 mL
STR Profile TH01: 7, 9.3 D5S818: 11, 12 D13S317: 11 D7S820: 9, 10 D16S539: 12,13 CSF1PO: 11,12Amelogenin: X,Y vWA: 17 TPOX:  8
Name of Depositor Susanne M. Gollin, University of Pittsburgh
Year of Origin February 1996
References

White JS, et al. The influence of clinical and demographic risk factors in the establishment of head and neck squamous cell carcinoma cell lines. Oral Oncol. 43:701-712, 2007. PubMed: 17112776

Martin CL, et al. Chromosomal imbalances in oral squamous cell carcinoma: examination of 31 cell lines and review of the literature. Oral Oncol. 44:369-382, 2008. PubMed: 17681875

Basic Documentation Product Sheet Certificate of Analysis SDS
Other Documentation Cell Micrograph
References

White JS, et al. The influence of clinical and demographic risk factors in the establishment of head and neck squamous cell carcinoma cell lines. Oral Oncol. 43:701-712, 2007. PubMed: 17112776

Martin CL, et al. Chromosomal imbalances in oral squamous cell carcinoma: examination of 31 cell lines and review of the literature. Oral Oncol. 44:369-382, 2008. PubMed: 17681875

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