JeKo-1; Mantle Cell Lymphoma; Human (Homo sapiens) Item ID: ATCCRL3006
| Permits and Restrictions |
View Permits |
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| Organism | Homo sapiens, human |
| Tissue | peripheral blood, Mantle Cell Lymphoma |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Lymphoma |
| Age | 78 |
| Gender | female |
| Applications | JeKo-1 cells are highly tumorigenic in SCID mice. RefJeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063 |
| Storage Conditions | liquid nitrogen vapor phase |
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| Derivation | The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. |
| Clinical Data | female The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. |
| Antigen Expression | CD3-, CD5+, CD10+, CD19+ (verified at ATCC) |
| Genes Expressed | CD3-, CD5+, CD10+, CD19+ (verified at ATCC) |
| Comments | The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. RefJeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063 JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. |
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| Subculturing | Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 104 to 4 X 104 viable cells/mL. Do not allow the cell concentration to reach 2.5 X 106 cells/mL. |
| Cryopreservation | Freeze medium: 30% RPMI; 60% FBS; 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: 5% CO2 in air recommended Temperature: 37°C |
| STR Profile | Amelogenin:X CSF1PO:9,12 D13S317:8,9 D16S539:12 D5S818:10,13 D7S820:10,11 THO1:7 TPOX:8 vWA: 14 |
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| Name of Depositor | E Campo |
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| References |
Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697 Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063 |