FSHR-18; anti-FSHR; Mouse (Mus musculus) Item ID: ATCCRL2688
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type | hybridoma: B lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human follicle stimulating hormone receptor (FSHR). |
| Storage Conditions | liquid nitrogen vapor phase |
| Derivation |
Animals were immunized with recombinant human follicle stimulating hormone receptor (FSHR) expressed in E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. |
|---|---|
| Genes Expressed | immunoglobulin; monoclonal antibody; against human follicle stimulating hormone receptor (FSHR), extracellular domain |
| Cellular Products | immunoglobulin; monoclonal antibody; against human follicle stimulating hormone receptor (FSHR), extracellular domain |
| Tumorigenic | Yes |
| Effects | Yes, forms ascites in BALB/c mice |
| Comments |
The antibody recognizes the N-terminal extracellular domain (amino acids domain 23-358) of the human follicle stimulating hormone receptor (FSHR) (hormone binding unit). The FSHR-18 (ATCC CRL-2688) and the FSHR-323 (ATCC CRL-2689) antibodies are additive. They recognize different epitopes. The FSHR-323 antibody inhibits hormone binding and the hormone-induced activation of adenylate cyclase but the FSHR-18 antibody does not. |
| Complete Growth Medium | Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10% |
|---|---|
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL.Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation | Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 37°C |
| Isotype | IgG1; kappa light chain |
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| Name of Depositor | H Loosfelt, E Milgrom |
|---|---|
| References |
Vannier B, et al. Anti-human FSH receptor monoclonal antibodies: immunochemical and immunocytochemical characterization of the receptor. Biochemistry 35: 1358-1366, 1996. PubMed: 8634264 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |