NL20-TA; Bronchus; Human (Homo sapiens) Item ID: ATCCRL2504
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Homo sapiens, human |
| Cell Type | Epithelial |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
2 Cells contain SV40 viral sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Accident victim |
| Age | 20 years |
| Gender | female |
| Ethnicity | Caucasian, White |
| Applications | NL20 (ATCC CRL-2503) is an immortalized, nontumorigenic human bronchial epithelial cell line derived from normal bronchus taken from an accident victim at autopsy. The cell line was established by transfection with the origin of replication-defective SV40 large T plasmid, p129. The non-tumorigenic NL20 cell line and the tumorigenic NL20-TA cell line form a pair of immortal cell lines that can be used to study tumor progression. |
| Derivation | NL20 (ATCC CRL-2503) is an immortalized, nontumorigenic human bronchial epithelial cell line derived from normal bronchus taken from an accident victim at autopsy. The cell line was established by transfection with the origin of replication-defective SV40 large T plasmid, p129. NL20 cells at passage 183 were inoculated into nude mice and a small slowly growing subcutaneous tumor developed from a minor clone in this otherwise stable cell line. |
|---|---|
| Clinical Data | female Caucasian, White 20 years |
| Tumorigenic | Yes |
| Effects | Yes, forms slowly growing tumors in nude mice |
| Comments | NL20 (ATCC CRL-2503) is an immortalized, nontumorigenic human bronchial epithelial cell line derived from normal bronchus taken from an accident victim at autopsy. The cell line was established by transfection with the origin of replication-defective SV40 large T plasmid, p129. NL20 cells at passage 183 were inoculated into nude mice and a small slowly growing subcutaneous tumor developed from a minor clone in this otherwise stable cell line. After three months the tumor was removed and placed in culture. At passage 3, these cells were re-injected into nude mice. One of the resulting tumors was dissociated, placed in culture and designated NL20-TA. This cell line (ATCC CRL-2504) remains tumorigenic up to at least passage 250. Neoplastic transformation of the NL20 cell line was associated with loss of chromosome 18 together with acquisition of multiple copies of 9q21.2-->34. The non-tumorigenic NL20 cell line and the tumorigenic NL20-TA cell line form a pair of immortal cell lines that can be used to study tumor progression. |
| Complete Growth Medium | Ham's F12 medium with 1.5 g/L sodium bicarbonate, 2.7 g/L glucose, 2.0 mM L-glutamine, 0.1 mM nonessential amino acids, 0.005 mg/ml insulin, 10 ng/ml epidermal growth factor, 0.001 mg/ml transferrin, 500 ng/ml hydrocortisone and 4% fetal bovine serum |
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| Subculturing | Subcultivation Ratio: A subcultivation ratio of 1:50 to 1:500 is recommended Medium Renewal: Every 2 to 3 days Remove medium, add dissociation solution (0.02% EDTA and 5% dialized fetal bovine serum in Ca-Mg free Hanks' BSS) and allow the flask to sit at 37C for 12 minutes or until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subculture weekly. |
| Cryopreservation | culture medium 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37.0°C |
| Name of Depositor | JH Schiller |
|---|---|
| Passage History | NL20 cells at passage 183 were inoculated into nude mice and a small slowly growing subcutaneous tumor developed from a minor clone in this otherwise stable cell line. After three months the tumor was removed and placed in culture. At passage 3, these cells were re-injected into nude mice. One of the resulting tumors was dissociated, placed in culture and designated NL20-TA. This cell line (ATCC CRL-2504) remains tumorigenic up to at least passage 250. |
| References |
Schiller JH, et al. Phenotypic, molecular and genetic characterization of transformed human bronchial epithelial cell strains. Int. J. Oncol. 4: 461-470, 1994. Schiller JH, Bittner G. Loss of the tumorigenic phenotype with in vitro, but not in vivo, passaging of a novel series of human bronchial epithelial cell lines: possible role of an alpha 5/beta 1-integrin-fibronectin interaction. Cancer Res. : 6215-6221, 1995. PubMed: 8521416 Schiller JH, et al. Karyotypic changes associated with spontaneous acquisition and loss of tumorigenicity in a human transformed bronchial epithelial cell line: evidence for in vivo selection of transformed clones. In Vitro Cell. Dev. Biol. Anim. 34: 283-289, 1998. PubMed: 9590501 |