In Vitro Technologies :: Products

Permits and Restrictions	View Permits
Organism	Homo sapiens, human
Cell Type	human papillomavirus 16 (HPV-16) transfected
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	2 Cells contain Human Papilloma viral 16 (HPV16) sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age	19 years
Gender	male
Applications	transfection host

Karyotype	diploid
Derivation	The ARPE/HPV-16 transformed cell line was derived from the ARPE-19 cell line (ATCC CRL-2302) by transfection with DH5-HPV-16. ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident.
Clinical Data	ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident. male
Antigen Expression	RPE-specific markers CRALBP and RPE-65 RefDunn KC, et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076
Genes Expressed	RPE-specific markers CRALBP and RPE-65
Comments	The ARPE/HPV-16 transformed cell line was derived from the ARPE-19 cell line (ATCC CRL-2302) by transfection with DH5-HPV-16. ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident. The cells express the RPE-specific markers CRALBP and RPE-65.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to culture vessels. Incubate cultures at 37C.
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C

STR Profile	Amelogenin: X, Y CSF1PO: 11 D13S317: 11, 12 D16S539: 9, 11 D5S818: 13 D7S820: 9, 11 THO1: 6, 9.3 TPOX: 9, 11 vWA: 16, 19

Name of Depositor	JW Obringer
References	Dunn KC, et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076

E: care@invitro.com.au
P: 1300 552 003

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