EOC 20; Macrophage; Mouse (Mus musculus) Item ID: ATCCRL2469
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Mus musculus, mouse |
| Tissue | brain |
| Cell Type | microglia |
| Product Format | frozen |
| Morphology | macrophage |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | 10 days juvenile |
| Gender | female |
| Strain | C3H/HeJ |
| Applications | The cells may be used to characterize the role of brain macrophages. |
| Derivation | This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. RefWalker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814 Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. |
|---|---|
| Clinical Data | female |
| Antigen Expression | CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD45 +, Ly-6C +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR +, F4/80 +/-, CD86 (B7.2) - |
| Receptor Expression | colony stimulating factor 1 (CSF-1R, CD115) |
| Comments |
Conditioned medium is made from LADMAC cells (ATCC CRL-2420) as a source of CSF-1. The cells exhibit phagocytic activity. These cells constitutively expressed high levels of major histocompatibility complex (MHC) class II antigens and expression was upregulated by recombinant murine interferon-gamma. C3H/HeJ strain is defective in TLR4 (toll-like receptor 4) Conditioned medium is made from LADMAC cells (ATCC CRL-2420 ) as a source of CSF-1. The cells exhibit phagocytic activity. |
| Complete Growth Medium | Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20% |
|---|---|
| Subculturing |
|
| Cryopreservation | culture medium 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37°C |
| Name of Depositor | WS Walker |
|---|---|
| Passage History | Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. |
| References |
Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247 Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530 Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814 Askew D, Walker WS. Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775 |