PMJ2-R Macrophage, Mus musculus ( mouse) Item ID: ATCCRL2458
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Mus musculus, mouse |
| Cell Type | peritoneal macrophage; infected with J2 viru |
| Product Format | frozen |
| Morphology | macrophage |
| Culture Properties | suspension; some adherent cells |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender | female |
| Strain | C57BL/6J |
| Applications | Cell staining demonstrated intracellular expression of the product of the v-raf gene in these cell lines. Analysis demonstrated the presence of peritoneal macrophage associated cell surface antigens, interleukin-6 (IL-6) secretion induced by lipopolysaccharide (LPS), and biological response modifier-induced cytotoxic activity against tumor cells. The cells are phagocytic, non-specific esterase positive and are Fc receptor positive. |
| Clinical Data | female |
|---|---|
| Antigen Expression | MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; F4/80 +; Ly-5 +; Lyt-1.1 -; Lyt-1.2 -; Thy-1.2 -; sIg - |
| Receptor Expression | Fc receptor (FcR) |
| Genes Expressed | MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; F4/80 +; Ly-5 +; Lyt-1.1 -; Lyt-1.2 -; Thy-1.2 -; sIg - |
| Comments | PMJ2-PC (ATCC CRL-2457) and AMJ2-R (ATCC CRL-2458) are cloned, continuous, peritoneal macrophage cell lines generated from C57BL6J mice by in vivo infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes. Cell staining demonstrated intracellular expression of the product of the v-raf gene in these cell lines. The in vivo immortalized cells had many of the morphological and functional characteristics of peritoneal macrophages. Analysis demonstrated the presence of peritoneal macrophage associated cell surface antigens, interleukin-6 (IL-6) secretion induced by lipopolysaccharide (LPS), and biological response modifier-induced cytotoxic activity against tumor cells. The cells are phagocytic, non-specific esterase positive and are Fc receptor positive. One of the clones, PMJ2-PC, constitutively expressed major histocompatibility complex (MHC) class II antigens, and in the other clone, PMJ2-R, MHC class II antigens expression was induced by recombinant murine interferon-gamma. |
| Complete Growth Medium | Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5% |
|---|---|
| Subculturing | Medium Renewal: Twice per week Scrape off the attached cells and transfer along with the floating cells into new flasks. |
| Cryopreservation | culture medium 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37.0°C |
| Name of Depositor | AV Palleroni |
|---|---|
| References |
Adami C, et al. In vivo immortalization of murine peritoneal macrophages: a new rapid and efficient method for obtaining macrophage cell lines. J. Leukocyte Biol. 53: 475-478, 1993. PubMed: 7683328 |