AMJ2-C8; Alveolar Macrophage; Mouse (Mus musculus) Item ID: ATCCRL2455
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Mus musculus, mouse |
| Tissue | lung |
| Cell Type | macrophage (alveolar); infected with J2 virus |
| Product Format | frozen |
| Morphology | macrophage |
| Culture Properties | suspension; some adherent cells |
| Biosafety Level |
2 [Cells contain J2 murine retrovirus viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | 10 weeks old |
| Gender | female |
| Strain | C57BL/6J |
| Applications | Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines. Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators. AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP. They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images | |
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| Clinical Data | female |
| Antigen Expression | MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; Ly-5 +; Thy-1 -; Lyt-1 - |
| Genes Expressed | MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; Ly-5 +; Thy-1 -; Lyt-1 - |
| Comments | AMJ2-C8 (ATCC CRL-2455) and AMJ2-C11 (ATCC CRL-2456) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes. Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines. Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators. AMJ2-C8 was activated following exposure to recombinant murine interferon gamma (rMuIFN-gamma) but not lipopolysaccharide (LPS) or muramyl dipeptide (MDP). AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP. The cells retain many characteristics of alveolar macrophages. They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors. |
| Complete Growth Medium | Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5% |
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| Subculturing | Scrape off the attached cells and transfer along with the floating cells into new flasks.Medium Renewal: Twice per week |
| Cryopreservation |
Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor | AV Palleroni |
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| References |
Palleroni AV, et al. Tumoricidal alveolar macrophage and tumor infiltrating macrophage cell lines. Int. J. Cancer 49: 296-302, 1991. PubMed: 1879973 Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |