LADMAC; Bone Marrow; Mouse (Mus musculus) Item ID: ATCCRL2420
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Mus musculus, mouse |
| Tissue | bone marrow |
| Cell Type | macrophage, monocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension, with some loosely adherent cells |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | adult |
| Strain | C3H |
| Applications | This cell line is used to produce LADMAC conditioned medium. It will support the growth of the macrophage cell lines EOC 2 (ATCC CRL-2467), EOC 13.31 (ATCC CRL-2468), EOC 20 (ATCC CRL-2469), I-11.15 (ATCC CRL-2470) and I-13.35 (ATCC CRL-2471). |
| Storage Conditions | liquid nitrogen vapor phase |
| Derivation | LADMAC is a transformed cell line derived by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with cloned human cellular myc-homologous sequences covalently attached to pBR325 (pR myc). |
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| Genes Expressed | colony stimulating factor-1 (CSF-1) |
| Cellular Products | colony stimulating factor-1 (CSF-1) |
| Tumorigenic | Yes |
| Effects | Yes, the cells are tumorigenic in nu+, nu+ mice but not in syngenic mice. |
| Comments |
The cell line has monocyte-like morphology; contains nonspecific esterase; is phagocytic for latex beads; secretes lysozyme, and bears the Mac-1 antigen.
A minority of cells are Fc receptor positive and an appreciable number of cells are complement receptor 1 positive. The cells are tumorigenic in nu+, nu+ mice but not in syngenic mice. The cells are not phagocytic for antibody or complement-coated particles; they do not constitutively secrete Interleukin-1. LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages. The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). Generation of conditioned Medium LADMAC conditioned medium is made from LADMAC cells (ATCC CRL-2420). LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages. The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1.RefOlivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247
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| Cryopreservation | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°C |
| Name of Depositor | WS Walker |
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| References |
Sklar MD, et al. Transformation of mouse bone marrow cells by transfection with a human oncogene related to c-myc is associated with the endogenous production of macrophage colony stimulating factor 1. J. Cell. Physiol. 125: 403-412, 1985. PubMed: 3877730 Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247 |