SVEC4-10EHR1; Vascular Endothelium; Mouse (Mus musculus) Item ID: ATCCRL2161
| Permits and Restrictions |
View Permits |
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| Organism | Mus musculus, mouse |
| Cell Type | endothelial, SV40 transformed |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | adult |
| Gender | male |
| Strain | C3H/HeJ |
| Applications | SVEC4-10EHR1 is an endothelial cell line derived from the same solid tumor as SVEC4-10EE2 (see ATCC CRL-2167) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181). SVEC4-10EHR1 expresses the cell surface major histocompatibility complex class I antigen (H-2 k) of the parental cell line, and express the vascular cell adhesion molecule (VCAM). |
| Derivation | SVEC4-10EHR1 is an endothelial cell line derived from the same solid tumor as SVEC4-10EE2 (see ATCC CRL-2167) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181). SVEC4-10EHR1 was cloned from cells from the SVEC4-10 induced tumor in 1990 by limiting dilution using medium conditioned by SVEC4-10EE2. |
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| Clinical Data | male |
| Antigen Expression | H-2 K; VCAM |
| Genes Expressed | H-2 K; VCAM |
| Tumorigenic | Yes |
| Effects | Yes, the cells induced nodular tumors in nude mice with some histopathologic characteristics of human Kaposi Sarcoma after a latency period of approximately 14 weeks |
| Comments | SVEC4-10EHR1 is an endothelial cell line derived from the same solid tumor as SVEC4-10EE2 (see ATCC CRL-2167) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181). SVEC4-10EHR1 was cloned from cells from the SVEC4-10 induced tumor in 1990 by limiting dilution using medium conditioned by SVEC4-10EE2. The cell line is distinguished by its spindle cell morphology both in vitro and in vivo in contrast to SVEC4-10EE2. SVEC4-10EHR1 expresses the cell surface major histocompatibility complex class I antigen (H-2 k) of the parental cell line, and express the vascular cell adhesion molecule (VCAM). The cells stain positively for SV40 T antigen. |
| Complete Growth Medium | Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10% |
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| Subculturing | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Twice per week Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Cryopreservation | Culture medium, 95%; DMSO, 5% |
| Name of Depositor | KA O'Connell |
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| References |
O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170 O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612 O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299 |
| Permits |
These permits may be required for shipping this product to Australia:
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| Basic Documentation | Product Sheet Certificate of Analysis SDS |
| References |
O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170 O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612 O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299 |