In Vitro Technologies

Permits and Restrictions	View Permits View Restrictions
Organism	Homo sapiens, human
Tissue	colon
Cell Type	Enterocyte
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	colorectal adenocarcinoma
Age	72 years
Gender	male
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase

Derivation	The C2BBe1 (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line (ATCC® HTB-37™) by limiting dilution. The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.
Clinical Data	male 72 years Caucasian
Receptor Expression	epidermal growth factor (EGF)
Comments	The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization. C2BBe1 cells form a polarized monolayer with an apical brush border (BB) morphologically comparable to that of the human colon. Isolated BB contained the microvillar proteins villin, fimbrin, sucrase-isomaltase, BB myosin-1 and the terminal web proteins fodrin and myosin II. The cells express substantial levels of BB myosin I similar to that of the human enterocyte. Although clonal, and far more homogeneous than the parental Caco-2 cell line with respect to BB expression, these cells are still heterogenous for microvillar length, microvillar aggregation, and levels of expression of certain BB proteins.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml human transferrin; fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subculture at 85% of confluence (about every 5 to 7 days). Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Twice per week
Cryopreservation	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37°C

STR Profile	Amelogenin: X CSF1PO: 11 D13S317: 11, 13, 14 D16S539: 12, 13 D5S818: 12, 13 D7S820: 11, 12 THO1: 6 TPOX: 9, 11 vWA: 16, 18

Name of Depositor	MD Peterson, M Mooseker
References	Basson MD, et al. Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994. PubMed: 8077287 Peterson MD, Mooseker MS. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. J. Cell Sci. 102: 581-600, 1992. PubMed: 1506435

Name of Depositor

MD Peterson, M Mooseker

References

Basson MD, et al. Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994. PubMed: 8077287

Peterson MD, Mooseker MS. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. J. Cell Sci. 102: 581-600, 1992. PubMed: 1506435

Permits	These permits may be required for shipping this product to Australia: For-profit customers must obtain a research use license agreement from the Contributor prior to shipment. DAFF Import Permit formerly known as AQIS Import Permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
FAQ's	C2BBe1 [clone of Caco-2] (ATCC® CRL-2102™) culture conditions Date Updated: 11/19/2015 Number of ATCC® CRL-2102 cells in a confluent flask Date Updated: 2/5/2013
Restrictions	NaviCyte Scientific holds the exclusive commercial distribution rights to the C2BBe1 cell deposited by Yale University with the American Type Culture Collection (ATCC). Note: All uses of ATCC® CRL-2102™, other than for research by a non-commercial or academic entity, require a license from NaviCyte Scientific under its exclusive arrangement with Memorial Sloan-Kettering Cancer Center. For information on the licensing terms, please contact NaviCyte Scientific via email or via phone at 973-868-6100.
References	Basson MD, et al. Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994. PubMed: 8077287 Peterson MD, Mooseker MS. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. J. Cell Sci. 102: 581-600, 1992. PubMed: 1506435

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C2BBe1 clone of Caco-2; Colon Adenocarcinoma; Human (Homo sapiens) Item ID: ATCCRL2102

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