CCD 1069SK HUMAN SKIN FIBROBLAST, NORMAL Item ID: ATCCRL2089
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Homo sapiens, human |
| Cell Type | fibroblast |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Carcinoma |
| Age | 70 years |
| Gender | female |
| Applications | The line was established from skin taken from normal breast tissue removed at mastectomy for invasive ductal carcinoma. |
| Derivation | The line was established from skin taken from normal breast tissue removed at mastectomy for invasive ductal carcinoma. |
|---|---|
| Clinical Data | female |
| Comments | The line was established from skin taken from normal breast tissue removed at mastectomy for invasive ductal carcinoma. Cells senesce after approximately 23 population doublings. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 3 to 4 days Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Cryopreservation | Culture medium, 95%; DMSO, 5% |