Sf9; Ovary; Fall Armyworm; (Spodoptera frugiperda) Item ID: ATCCRL1711
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Spodoptera frugiperda, fall armyworm |
| Tissue | ovary |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | mixed: adherent/suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | pupa |
| Gender | female |
| Applications | This line can be used to replicate baculovirus expression vectors. This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images | |
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| Clinical Data | female |
| Virus Susceptibility | Nuclear polyhedrosis virus, Autographa californica St. Louis encephalitis virus , St. Louis encephalitis virus |
| Comments | It is important to use the medium described below. |
| Complete Growth Medium | The base medium for this cell line is Grace's Insect Medium Supplemented (GIBCO/Invitrogen Cat. No. 11605-094 or equivalent). To make the complete growth medium, add the following components to the base medium: heat-inactivated insect cell culture tested fetal bovine serum to a final concentration of 10%. |
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| Subculturing | Gently resuspend cells in the spent culture medium by pipetting across the monolayer or by hitting the flask against the palm of your hand (the latter is only preferable when working with larger flasks). Subcultivation Ratio: A subcultivation ratio of 1:5 or greater is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 100% Temperature: 28°C Growth Conditions: The recommended media are formulated for use without CO2. Omission of the yeastolate or lactalbumin hydrolysate will lead to poor performance by this line. |
| Name of Depositor | G Smith, C Cherry, MD Summers |
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| Year of Origin | 1983 |
| References |
Vaughn JL, et al. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13: 213-217, 1977. PubMed: 68913 Zhang PF, et al. Susceptibility of the Sf9 insect cell line to infection with adventitious viruses. Biologicals 22: 205-213, 1994. PubMed: 7811453 Smith GE, et al. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc. Natl. Acad. Sci. USA 82: 8404-8408, 1985. PubMed: 3878519 Hall MR, Gibson W. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzym e when separately cloned and coexpressed in eukaryotic cells. J. Virol. 70: 5395-5404, 1996. PubMed: 8764050 Bakker A, et al. Human T-cell leukemia virus type 2 Rex inhibits pre-mRNA splicing in vitro at an early stage of spliceosome formation. J. Virol. 70: 5511-5518, 1996. PubMed: 8764063 Gibson W, et al. Cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus UL46. J. Virol. 70: 7454-7461, 1996. PubMed: 8892863 Filtz TM, et al. Purification and G protein subunit regulation of a phospholipase C-beta from Xenopus laevis oocytes. J. Biol. Chem. 49: 31121-31126, 1996. PubMed: 8940109 Lindorfer MA, et al. G protein gamma subunits with altered prenylation sequences are properly modified when expressed in Sf9 cells. J. Biol. Chem. 271: 18582-18587, 1996. PubMed: 8702508 Bruant SS, et al. N-terminal sequences contained in the Src homology 2 qand 3 domains of p120 GTPase-activating protein are required for full catalytic activity toward ras. J. Biol. Chem. 271: 5195-5199, 1996. PubMed: 8617802 Stewart L, et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601, 1996. PubMed: 8631793 Kawabe J, et al. Soluble adenylyl cyclase from Spodoptera frugiperda (Sf9) cells. J. Biol. Chem. 271: 20132-20137, 1996. PubMed: 8702736 Yasuda H, et al. Role of the prenyl group on the G protein gamma subunit in coupling trimeric G proteins to A1 adenosine receptors. J. Biol. Chem. 271: 18588-18595, 1996. PubMed: 8702509 Sf9 was cloned by G. E. Smith and C. L. Cherry from the parent line, IPLB-SF 21 AE, which was derived from pupal ovarian tissue of the fall armyworm, Spodoptera frugiperda, by Vaughn, et al., in 1977. |