LL/2 (LLC1); Lewis Lung Carcinoma; Mouse (Mus musculus) Item ID: ATCCRL1642
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Mus musculus, mouse |
| Tissue | lung |
| Product Format | frozen |
| Morphology | rounded - loosely attached or floating |
| Culture Properties | mixed, adherent and suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Lewis lung carcinoma |
| Strain | C57BL |
| Applications | This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents. |
| Storage Conditions | liquid nitrogen vapor phase |
| Derivation | Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma. |
|---|---|
| Antigen Expression | H-2b |
| Tumorigenic | Yes |
| Effects | Yes, in C57BL mice |
| Comments | The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate. The cells are reported to be highly tumorigenic, but weakly metastatic in mice. The cells form multilayers in flasks without actually becoming confluent. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing | Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| Population Doubling Time | 21 hrs |
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| Name of Depositor | JS Bertram |
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| References |
Bertram JS, Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett. 11: 63-73, 1980. PubMed: 7226139 Sharma S, et al. T cell-derived IL-10 promotes lung cancer growth by suppressing both T cell and APC function. J. Immunol. 163: 5020-5028, 1999. PubMed: 10528207 |