CCD-18Co; Colon; Human (Homo sapiens) Item ID: ATCCRL1459
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Homo sapiens, human |
| Tissue | colon |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | 2.5 months |
| Gender | female |
| Ethnicity | Black |
| Storage Conditions | liquid nitrogen vapor temperature |
| Images | |
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| Clinical Data | 2.5 months Black female |
| Comments | The line begins to senesce at about PDL = 42. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing | Remove medium, and rinse with 0.25% trypsin, 0.053 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions | Temperature: 37°C Growth Conditions: Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium. |
| STR Profile | Amelogenin: X CSF1PO: 8 D13S317: 12 D16S539: 12,13 D5S818: 12 D7S820: 8 THO1: 6,7 TPOX: 8,11 vWA: 15,17 |
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| Population Doubling Time | The line begins to senesce at about PDL = 42. |
| Name of Depositor | MI Cour |
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| References |
Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 Hinterleitner TA, et al. Il-1 stimulates intestinal myofibroblast COX gene expression and augments activation of CI- secretion in T84 cells. Am. J. Physiol. 271: C1262-C1268, 1996. PubMed: 8897833 |