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PWR-1E; Prostate Epithelium; Human (Homo sapiens) Item ID: ATCCRL11611

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Organism Homo sapiens, human
Tissue prostate
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain Adenovirus 12 and SV40 DNA viral sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease normal
Age 67 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype At passage 42, a majority of the cells were in the 56-59 range; XY RefWebber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420
Derivation Tumor Suppressor gene(s):p53 + [PubMed: 8761420] Human prostatic epithelial cells, derived from a normal prostate with mild hyperplasia, were immortalized with an adenovirus 12-SV40 hybrid virus (Ad12-SV40).

PWR-1E cell line (ATCC CRL-11611) was derived by single cell cloning of a non-producer cell [PubMed: 8761420].

 
Clinical Data 67 years male
Antigen Expression kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen)
Receptor Expression androgen receptor, expressed RefWebber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420(upregulated upon exposure to androgen)
Genes Expressed cytokeratin 18,cytokeratin 8,SV40 large T antigen,kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen)
Cellular Products cytokeratin 18 cytokeratin 8 SV40 large T antigen
Tumorigenic No
Effects No, in nude mice (with or without Matrigel) Yes, the cells do form colonies in soft agar
Comments Tumor Suppressor gene(s): p53 + [PubMed: 8761420] Human prostatic epithelial cells, derived from a normal prostate with mild hyperplasia, were immortalized with an adenovirus 12-SV40 hybrid virus (Ad12-SV40).

PWR-1E cells organize into acini and secrete PSA into the lumen, when exposed to androgen.

PWR-1E cells organize into acini and secrete PSA into the lumen, when exposed to androgen. When injected with Matrigel or with stromal cells, into athymic male rodents, PWR-1E cells also organize into acini and produce PSA [Webber MM, personal communication].

 

 
Complete Growth Medium The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit
  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
    • Subculturing Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS).
    3. Add 2.0 to 3.0 mL (to a T-25 flask) or 3.0 to 4.0 mL (to a T-75 flask) of 0.05% Trypsin - 0.53mM EDTA solution, diluted 1:1 with D-PBS, and place flask in a 37C incubator for 5 to 8 minutes. Observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
    4. Add 6.0 to 8.0 mL of 0.1% Soybean Trypsin Inhibitor (ATCC® 30-2014™) or 2% fetal bovine serum in D-PBS, as appropriate, and aspirate cells by gently pipetting.
    5. Transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 7 minutes.
    6. Discard supernatant and resuspend cells in fresh serum-free growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 2 X 104 to 4 X 104 viable cells/cm2 is recommended.
    7. Incubate cultures at 37°C. We recommend that you maintain cultures at a cell concentration between 4 X 104 and 7 X 104 cells/cm2.
    Cells grown under serum-free or reduced serum conditions may not attach strongly during the 24 hours after subculture and should be disturbed as little as possible during that period.  Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended Medium Renewal: Every 2 days
    Cryopreservation Freeze Medium: Complete growth medium supplemented with 7.5% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase
    Culture Conditions Atmosphere: Air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.
    STR Profile Amelogenin: X,Y CSF1PO: 10,12 D13S317: 12 D16S539: 12,13 D5S818: 11,13 D7S820: 9,11 THO1: 8,9.3 TPOX: 8,11 vWA: 14,16
    Isoenzymes AK-1, 1 RefWebber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420 ES-D, 1 RefWebber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420 G6PD, B RefWebber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420 GLO-I, 2 RefWebber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420 Me-2, 0 RefWebber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420 PGM1, 1 RefWebber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420 PGM3, 1 RefWebber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420
    Name of Depositor Michigan State University, National Cancer Institute
    U.S. Patent Number 5,610,043
    Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
    References

    Webber MM, Rhim JS. Human prostatic cell lines immortalized by adenovirus 12-simian virus 40 (AD12/SV40) hybrid virus. US Patent 5,610,043 dated Mar 11 1997

    Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420

    Okamoto M, et al. Interleukin-6 and epidermal growth factor promote anchorage-independent growth of immortalized human prostatic epithelial cells treated with N-methyl-N-nitrosourea. Prostate 35: 255-262, 1998. PubMed: 9609548

    Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337

    Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152

    Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497

    upregulated upon exposure to androgen

    Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420

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