Vero-SF-ACF; Kidney; African Green Monkey (Cercopithecus aethiops) Item ID: ATCCCL815
| Permits and Restrictions |
View Permits |
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| Organism | Cercopithecus aethiops |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | adult |
| Applications | detection of verotoxin efficacy testing malaria biology media testing mycoplasma testing substrate testing transfection host detection of virus in ground beef |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | This is a cell line with the hypodiploid chromosome count. The modal chromosome number was 58 occurring in 66% of cells. In most cells, over 50% of the chromosomes in each cell complement belonged to structurally altered marker chromosomes. Normal A3, A4, B4, and B5 were absent; B2, B3 and B7 were occasionally paired; and B9, C1 and C5 were mostly paired. The rate of cells with higher ploidies was 1.7%. Other chromosomes were mostly present in single copy. |
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| Images | |
| Derivation | The parental Vero cell line was initiated from the kidney of a normal adult African green monkey on March 27, 1962, by Y. Yasumura and Y. Kawakita at the Chiba University in Chiba, Japan |
| Comments |
Vero-SF-ACF cell line was derived from the parental Vero cell line (ATCC CCL-81) by adaptation to serum-free and animal component-free medium. The parental Vero cell line has been shown to be susceptible to infection by: poliovirus 1, 2, 3; Getah; Ndumu; Pixuna; Ross River; Semliki Forest; Paramaribo; Kokobera; Modoc; Murutucu; Germiston; Guaroa; Pongola; Tacaribe; SV-5; SV40; rubeola; rubellavirus; reovirus 1, 2, 3; and simian adenoviruses. The parental Vero cell line has been show to be resistant to infection by the following viruses: Stratford; Apeu; Caraparu; Madrid; Nepuyo; Ossa |
| Complete Growth Medium |
These cells may be grown in VP-SFM (Cat. No. 11681-020, Life Technologies) according to the manufacturer's instructions. |
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| Subculturing |
Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1.3 to 1:6 Medium Renewal: One to two times weekly |
| Cryopreservation |
Freeze medium:
Serum-Free Cell Freezing Medium (ATCC 30-2600) Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Name of Depositor | ATCC |
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| Year of Origin | 2011 |
| References |
Yasumura Y, Kawakita Y. Studies on SV40 in tissue culture - preliminary step for cancer research in vitro. Nihon Rinsho 21: 1201-1215, 1963. Simizu B, et al. Characterization of the Tacaribe group of arboviruses. I. Propagation and plaque assay of Tacaribe virus in a line of African green monkey kidney cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 119-123, 1967. PubMed: 6027511 Rhim JS, Schell K. Cytopathic and plaque assay of rubella virus in a line of African green monkey kiency cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 602-606, 1967. PubMed: 4961492 Rhim JS, et al. Temperature dependence of the synthesis of adenovirus tumor and viral antigens. Proc. Soc. Exp. Biol. Med. 127: 642-646, 1968. PubMed: 5689485 Rhim JS, Schell K. Cytopathic effects of the parainfluenza virus SV5 in Vero cells. Nature 216: 271-272, 1967. PubMed: 4293683 Rhim JS, et al. Growth of Junin virus, the etiological agent of Argentinian hemorrhagic fever, in cell cultures. Arch. Gesamte Virusforsch. 21: 243-252, 1967. PubMed: 5591575 Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999. |