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RK13; Kidney; Rabbit (Oryctolagus cuniculus) Item ID: ATCCCL37

Permits and Restrictions

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Organism Oryctolagus cuniculus, rabbit
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain Bovine Viral Diarrhea Virus (BVDV)]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease normal
Age 5 weeks
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Genes Expressed keratin
Cellular Products keratin
Virus Susceptibility Herpes simplex virus Pseudorabies virus Vaccinia virus Rabbitpox virus Myxoma virus Simian adenovirus 11 Simian adenovirus 17 Simian adenovirus 3 Simian adenovirus 1 Simian adenovirus 20 Simian adenovirus 18 Simian adenovirus 16 Simian adenovirus 8 Simian adenovirus 19 Simian adenovirus 21 Simian adenovirus 25 Simian adenovirus 22 Simian adenovirus 23 Simian adenovirus 38 Simian adenovirus 37 Simian adenovirus 27 Simian adenovirus 27 Simian adenovirus 39 Simian adenovirus 32 Simian adenovirus 34 Simian adenovirus 31 Simian adenovirus 33 Simian adenovirus 36 Rubella virus , Rubella virus
Comments

The cells are positive for keratin by immunoperoxidase staining. The cells are positive for bovine viral diarrhea virus (BVDV).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: Twice per week
Cryopreservation Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C
Name of Depositor BC Meyer
References

Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

Beale AJ, et al. Rabbit Cells Susceptible to Rubella Virus. The Lancet 282: 640-641 1963

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

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