Permits and Restrictions |
View Permits View Restrictions |
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Organism | Homo sapiens, human |
Tissue | bone marrow CD34+ cells |
Cell Type | neural progenitor cells |
Product Format | frozen 1.0 mL per vial |
Morphology | short spindle shape |
Culture Properties | adherent |
Biosafety Level |
2 [Cells contain Sendai viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease | normal |
Age | 27 |
Gender | female |
Ethnicity | Chinese |
Applications | Neuronal differentiation and drug screen |
Storage Conditions | Liquid nitrogen vapor phase (-130°C or colder) |
Images | |
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Derivation | Normal NPCs (ATCC® ACS-5003™) are derived from ATCC-BXS0117 Human [Asian Female] Induced Pluripotent Stem Cells (ATCC®ACS-1031™) |
Antigen Expression | Nestin, Pax-6 |
Comments | Astrocyte, oligodendrocyte, and neuron differentiation; drug screening |
Complete Growth Medium |
Complete growth media for Neural Progenitor Cells (NPCs) includes DMEM: F12 (ATCC®30-2006) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC®ACS-3003). To make complete NPC medium add the following components of the kit to 464 mL DMEM: F12: 5 mL L-Alanyl-L-Glutamine 5 mL Non-Essential Amino Acids 10 mL NPC Growth Kit Component A 5 mL NPC Growth Kit Component B 1 mL NPC Growth Kit Component C 10 mL NPC Growth Kit Component D |
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Seeding Density | Post-thaw: 80,000 viable cells/cm2 on CellMatrix-coated dishes/plates.Subculture: 40,000 viable cells/cm2 on CellMatrix-coated dishes/plates. |
Subculturing |
Post thaw day 1, perform a 100% medium change and remove all cells that did not attach. Perform a 100% medium change every other day thereafter. Passage the cells cells with diluted Accutase (50% Accutase and 50% DPBS) when they reach ~95% confluence and reseed the NPCs at 40,000 viable cells/cm2 on CellMatrix-coated dishes/plates. |
Cryopreservation | Stem Cell Freezing Media (ATCC® ACS-3020™) |
Culture Conditions |
Coat plates with CellMatrix (ATCC®ACS-3035) and culture the NPCs with NPC Growth Medium (ATCC® ACS-3003). |
Cells per Vial | >1,000,000 cells per vial |
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Volume | 1.0 mL per vial |
STR Profile | Amelogenin: X CSF1PO: 10,12 D13S17: 9,12 D16S539: 11 D5S818: 12 D7S820: 9,13 TH01: 7,9 TPOX: 8,11 vWA: 19 |
Sterility Tests |
No bacterial or fungal growth after 21 days |
Viral Testing | None detected |
Viability | ≥80% viability |
Functional Tests | Exhibit differentiation potential of NPCs into Tuj1+ early neurons and TH+ dopaminergic neurons |
Passage Number | P4 |
Population Doubling Capacity | ≥15 PDLs |
Population Doubling Time | ~38 hours |
Name of Depositor | ATCC |
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Year of Origin | 2015 |
References |
Malik N, et al. Compounds with species and cell type specific toxicity identified in a 2000 compound drug screen of neural stem cells and rat mixed cortical neurons. Neurotoxicology 45: 192-200, 2014. PubMed: 25454721 |