Questions? Feedback? powered by Olark live chat software
Permits and Restrictions

View Permits View Restrictions

Organism Homo sapiens, human
Tissue bone marrow CD34+ cells
Cell Type neural progenitor cells
Product Format frozen 1.0 mL per vial
Morphology short spindle shape
Culture Properties adherent
Biosafety Level 2  [Cells contain Sendai viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 27
Gender female
Ethnicity Chinese
Applications Neuronal differentiation and drug screen
Storage Conditions Liquid nitrogen vapor phase (-130°C or colder)
Images
Derivation Normal NPCs (ATCC® ACS-5003™) are derived from ATCC-BXS0117 Human [Asian Female] Induced Pluripotent Stem Cells (ATCC®ACS-1031™)
Antigen Expression Nestin, Pax-6
Comments Astrocyte, oligodendrocyte, and neuron differentiation; drug screening
Complete Growth Medium

Complete growth media for Neural Progenitor Cells (NPCs) includes DMEM: F12 (ATCC®30-2006) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC®ACS-3003). To make complete NPC medium add the following components of the kit to 464 mL DMEM: F12:

5 mL L-Alanyl-L-Glutamine 5 mL Non-Essential Amino Acids 10 mL NPC Growth Kit Component A 5 mL NPC Growth Kit Component B 1 mL NPC Growth Kit Component C 10 mL NPC Growth Kit Component D
Seeding Density Post-thaw: 80,000 viable cells/cm2 on CellMatrix-coated dishes/plates.Subculture: 40,000 viable cells/cm2 on CellMatrix-coated dishes/plates.
Subculturing

Post thaw day 1, perform a 100% medium change and remove all cells that did not attach. Perform a 100% medium change every other day thereafter. Passage the cells cells with diluted Accutase (50% Accutase and 50% DPBS) when they reach ~95% confluence and reseed the NPCs at 40,000 viable cells/cm2 on CellMatrix-coated dishes/plates.

Cryopreservation Stem Cell Freezing Media (ATCC® ACS-3020™)
Culture Conditions

Coat plates with CellMatrix (ATCC®ACS-3035) and culture the NPCs with NPC Growth Medium (ATCC® ACS-3003).

Cells per Vial >1,000,000 cells per vial
Volume 1.0 mL per vial
STR Profile Amelogenin: X CSF1PO: 10,12 D13S17: 9,12 D16S539: 11 D5S818: 12 D7S820: 9,13 TH01: 7,9 TPOX: 8,11 vWA: 19
Sterility Tests

No bacterial or fungal growth after 21 days

Viral Testing None detected
Viability ≥80% viability
Functional Tests Exhibit differentiation potential of NPCs into Tuj1+ early neurons and TH+ dopaminergic neurons
Passage Number P4
Population Doubling Capacity ≥15 PDLs
Population Doubling Time ~38 hours
Name of Depositor ATCC
Year of Origin 2015
References

Malik N, et al. Compounds with species and cell type specific toxicity identified in a 2000 compound drug screen of neural stem cells and rat mixed cortical neurons. Neurotoxicology 45: 192-200, 2014. PubMed: 25454721

E: care@invitro.com.au
P: 1300 552 003