Euglena gracilis Z Item ID: ATC12716
| Permits and Restrictions |
View Permits |
|---|---|
| Strain Designations | Z |
| Application | assay of vitamin B12 cobalamin, cobalamins |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation | freshwater material from Saedeleer, 1950 |
| Product Format | frozen |
| Type Strain | no |
| Comments | Medium for Vitamin B12 assay Regulation of cell shape Growth and cell volume Aromatic amino acid pathway water-soluble vitamins in cells and spent culture supernatants Molecular biology Synchronization of division cryopreservation photosynthetic |
|---|
| Medium | ATCC® Medium 351: Hutner's medium for Euglena |
|---|---|
| Growth Conditions | Temperature: 25.0°C Duration: axenic |
| Cryopreservation |
1. Harvest cells from a culture which is at or near peak density by centrifuging at 100 x g for 1 minute. |
| Name of Depositor | SH Hutner |
|---|---|
| Chain of Custody | ATCC |
| Year of Origin | 1950 |
| References |
Byng GS, et al. The aromatic amino acid pathway branches at L-arogenate in Euglena gracilis. Mol. Cell. Biol. 1: 426-438, 1981. PubMed: 6152855 Shehata TE, Kempner ES. Growth and cell volume of Euglena gracilis in different media. Appl. Environ. Microbiol. 33: 874-877, 1977. PubMed: 405924 Hutner SH, et al. A sugar-containing basal medium for vitamin B12-Assay with Euglena; Application to body fluids. J. Protozool. 3: 101-112, 1956. Lonergan TA, Williamson LC. Regulation of cell shape in Euglena gracilis. V. Time-dependent responses to Ca2+ agonists and antagonists. J. Cell Sci. 89: 365-371, 1988. PubMed: 3143739 Analytical microbiology. New York: Academic Press; 1963. Analytical microbiology. New York: Academic Press; 1963. Hwang SW, Hudock GA. Stability of Chlamydomonas reinhardi in liquid nitrogen storage. J. Phycol. 7: 300-303, 1971. Hwang SW, Horneland W. Survival of algal cultures after freezing by controlled and uncontrolled cooling. Cryobiology 1: 305-311, 1965. PubMed: 5872223 Baker ER, et al. Water-soluble vitamins in cells and spent culture supernatants of Poteriochronous stipitata, Euglena gracilis and Tetrahymena thermophila. Arch. Microbiol. 129: 310-313, 1981. Kempner ES, Miller JH. The molecular biology of Euglena gracilis. IX. Amino acid pool composition. J. Protozool. 21: 363-367, 1974. PubMed: 4209142 Shehata TE, Kempner ES. Synchronization of division in vitamin B12-starved Euglena gracilis. J. Protozool. 26: 626-630, 1979. PubMed: 120896 |