Pseudomonas aeruginosa; GFP Item ID: ATC10145GFP
| Permits and Restrictions |
View Permits View Restrictions |
|---|---|
| Application | Fluorescence detection Fluorescence imaging FACS Cell infection assaysIn vivo localization Pathogen-host interactions assays |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen |
| Storage Conditions | -80°C or colder |
| Preceptrol® | no |
|---|---|
| Type Strain | no |
| Antibiotic Resistance | Ampicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 300 µg/mL ampicillin. |
| Comments | This clone was derived from ATCC® 10145™ and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the Plac promoter. This construct has been designed for Gram-negative bacteria fluorescence labeling.Vector: pUCP18-MCSgfpmut3Vector size: 5.492kbVector type: plasmidMarker: bla (ampR)Features:GFPmut3: fluorescent maker under the control of Plac promoterOrigin of replication: ori from pRO1600Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoIDouble restriction sites: HindIII, PstI, SalI, XbaI |
| Medium | ATCC® Medium 2854: Nutrient Agar/Broth with 300 mcg/ml Ampicillin |
|---|---|
| Growth Conditions | Temperature: 37°CAtmosphere: Aerobic |
| Name of Depositor | M Barbier |
|---|---|
| Chain of Custody | Modified by ATCC, Dr. M. Barbier <-- ATCC <-- F Kavanagh <-- Merck Sharp & Dohme |
| References |
Barbier M and Mittar D. Novel fluorescent reporters for studying pathogen-host interactions. ASM general meeting 2014 Poster. Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227 Opinion 36. Designation of strain ATCC 10145 as the neotype strain of Pseudomonas aeruginosa (Schroeter) Migula. Int. J. Syst. Bacteriol. 20: 15-16, 1970. Hugh R, Leifson E. The proposed Neotype Strains of Pseudomonas aeruginosa (Schroeter 1872) Migula 1900. Int. Bull. Bacteriol. Nomencl. Taxon 14: 69-84, 1964. |